Heterogeneity and variation among Neisseria meningitidis lipopolysaccharides.

Journal of Bacteriology
C M TsaiC E Frasch

Abstract

Eight immunotype lipopolysaccharides (LPSs) of Neisseria meningitidis were prepared by the phenol-water procedure and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and sugar analyses. By SDS-PAGE and a highly sensitive silver strain. N. meningitidis LPSs from cells grown in tryptic soy broth were shown to contain one or two predominant components and a few minor, somewhat higher-molecular-weight components. The molecular sizes of the two predominant components were approximately the same as those of two E. coli rough-type LPSs, one with a complete core and the other with an incomplete core. The molecular weight of the major LPS component varied somewhat among different immunotypes but was estimated to be in the range of 4,200 to 5,000. By sugar analyses, the eight immunotype LPSs were different in their monosaccharide compositions. All contained glucose, galactose, heptose, glucosamine, and 2-keto-3-deoxyoctonate, but in different molar ratios. The growth of N. meningitidis in tryptic soy broth under different levels of aeration resulted in a change in the two major LPS components seen on the SDS-PAGE gel. High aeration increased the amount of the smaller component, whereas low aeration i...Continue Reading

References

Jul 1, 1979·The Journal of Infectious Diseases·M A Apicella
Sep 1, 1977·Bacteriological Reviews·I OrskovK Jann
May 1, 1977·Infection and Immunity·R E Mandrell, W D Zollinger
Feb 1, 1978·Journal of General Microbiology·G A LimjucoD J Carlo
Jan 1, 1976·Canadian Journal of Microbiology·K G Johnson, M B Perry
Jul 1, 1974·The Journal of Experimental Medicine·C E Frasch, E C Gotschlich
Nov 1, 1972·Infection and Immunity·P C MaloneyB L Brandt
Feb 1, 1980·Canadian Journal of Biochemistry·H J JenningsG Calver
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1980·Journal of Biochemical and Biophysical Methods·R A Boykins, T Y Liu
Sep 1, 1953·Bacteriological Reviews·S E BRANHAM
Sep 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·M J OSBORN
Apr 1, 1964·The Australian Journal of Experimental Biology and Medical Science·F M COLLINS

❮ Previous
Next ❯

Citations

Jun 1, 1995·Molecular Microbiology·J P van Putten, B D Robertson
Jan 15, 1999·Critical Reviews in Microbiology·C M Kahler, D S Stephens
Jan 19, 2006·Clinical Microbiology Reviews·Lee H Harrison
Oct 1, 1984·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·E Rødahl, J A Maeland
Dec 20, 2008·The Journal of Biological Chemistry·Chao-Ming TsaiJohn F Cipollo
Jan 1, 1996·Critical Reviews in Microbiology·A PrestonM A Apicella
Jul 26, 2012·Cellular Microbiology·Paola QuattroniRachel M Exley
Sep 1, 1996·Progress in Lipid Research·S G Wilkinson
Apr 1, 1984·Journal of Clinical Microbiology·T R Parr, L E Bryan
Feb 1, 1990·Journal of Clinical Microbiology·M VaneechoutteA M Van Den Abeele
Oct 1, 1986·Infection and Immunity·R MandrellJ M Griffiss
Jun 1, 1986·Journal of Bacteriology·P J HitchcockD C Morrison

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.