PMID: 1324774Jul 10, 1992

Heterogeneity in EC50 and nH of GABAA receptors on dorsal root ganglion neurons freshly isolated from adult rats

Brain Research
G White

Abstract

GABA activates a Cl- current through the GABAA receptor/ionophore complex that influences excitability of neurons. Studies using expression of cloned cDNAs coding for different GABAA receptor/ionophore subunits suggest that the EC50 and Hill coefficient for GABA are influenced by subunit composition. However, no direct evidence for such heterogeneity has been reported for vertebrate neurons. I have investigated the heterogeneity of EC50 and Hill coefficients (nH) of isolated dorsal root ganglion neurons using the whole-cell patch clamp technique. The EC50 for GABA varied from 26 to 107 microM among neurons. nH calculated from the logistic equation varied from 1.18 to 2.0. A negative correlation was found between the EC50 and nH (r = -0.81). Both nH and EC50 differed between some cells. However, in some instances, nH differed between cells while EC50 values were similar, and in other cells, EC50 values differed and nH was similar. In addition, when cells were categorized according to action potential shape, the EC50 and Hill coefficients differed among cell types in some instances and were similar in other instances. These findings demonstrate that different pharmacological profiles for GABA can be observed in adult mammalian ne...Continue Reading

References

Mar 5, 1998·Neuroscience Letters·M D KrasowskiG E Homanics
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·G E HomanicsR W Olsen
Oct 17, 1998·Neuropharmacology·A V WilliamsonA D Randall
Feb 14, 2015·Cell·Christina HanackJan Siemens
Feb 21, 2006·The European Journal of Neuroscience·H HayasakiM Watanabe
Apr 14, 1999·Journal of Neurophysiology·E I TietzR L Macdonald

Citations

Related Concepts

Vertebrates
August Rats
Neurons
Action Potentials
PDZK1 gene
Entire Spinal Nerve Ganglion
Clamping Activity
Neuronal
Ionophores
GABA-A Receptor gamma Subunit

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