PMID: 16505581Mar 1, 2006Paper

Heterogeneity in expression and subcellular localization of tight junction proteins, claudin-10 and -15, examined by RT-PCR and immunofluorescence microscopy

Archives of Histology and Cytology
Tetsuichiro InaiYosaburo Shibata

Abstract

Tight junctions regulate paracellular permeability, create the luminal fluid microenvironment of blood vessels and the digestive tract, and also form the protective barrier in the stratified epithelium including the epidermis. Claudins are the integral membrane proteins at tight junctions and form a multigene family composed of at least 24 members, but knowledge of the subcellular localization of each claudin is still fragmentary. We performed RT-PCR for fifteen claudin species to examine the mRNA expression in various mouse tissues, and focused on investigating the subcellular localization of claudin-10 and -15 by immunofluorescence microscopy in various rat tissues. Neither claudin-10 nor -15 was detected in vascular endothelial cells in most tissues, and these claudins were restricted to the vasa recta in the kidney medulla. Both claudins were also detected at apical tight junctions in the epithelium of the jejunum with no intensity gradients along the crypt-to-villus axis. However, both claudins were expressed only in the basal half of the crypt epithelium in the colon, showing obvious gradients along crypt-to-surface axis. Moreover, claudin-10 showed the ectopic subcellular localization where tight junction strands do not ...Continue Reading

References

Jan 24, 1977·Cell and Tissue Research·P MottaS Correr
Dec 1, 1975·The Journal of Cell Biology·M SimionescuG E Palade
Jan 1, 1982·Annals of the New York Academy of Sciences·E E Schneeberger
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MoritaS Tsukita
Apr 4, 2000·The Journal of Cell Biology·S Tsukita, M Furuse
Nov 22, 2000·The Journal of Cell Biology·A ZahraouiT Galli
Feb 13, 2001·Placenta·J F DyeJ A Firth
Jun 18, 2002·European Journal of Cell Biology·Johanna M BrandnerIngrid Moll
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Minghui QinBehrooz G Sharifi
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
Aug 12, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carolyn B CoyneLarry G Johnson
Dec 31, 2003·Hearing Research·Shin-iciro KitajiriShoichiro Tsukita
Jan 15, 2004·American Journal of Physiology. Renal Physiology·Wing Y LiAlan S L Yu
Apr 8, 2004·American Journal of Physiology. Renal Physiology·Prasad AcharyaGerard Apodaca
Mar 4, 2005·Breast Cancer Research : BCR·Brigitte BlackmanMargaret C Neville
May 24, 2005·Mechanisms of Development·Tammy-Claire TroyKursad Turksen
Nov 9, 2005·Archives of Histology and Cytology·Xin GuanYosaburo Shibata
May 11, 2006·Skin Pharmacology and Physiology·J M BrandnerI Moll

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Citations

Nov 9, 2007·Histochemistry and Cell Biology·Akihito SengokuYosaburo Shibata
Sep 25, 2012·Journal of Molecular Histology·Kannikar WongdeeNarattaphol Charoenphandhu
Jul 19, 2011·Molecular Biology Reports·P N GabrovskaL R Griffiths
Feb 4, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Adam KirkJane Collins
Feb 26, 2010·Journal of Biomedicine & Biotechnology·Olga J Baker
Sep 2, 2008·American Journal of Respiratory Cell and Molecular Biology·Anna C ZemkeBarry R Stripp
Nov 9, 2005·Archives of Histology and Cytology·Xin GuanYosaburo Shibata
May 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Christiane KirchhoffAnnemarie Samalecos
Apr 19, 2012·Gastroenterology·Daniel DirenzoStephen F Konieczny
Jun 28, 2011·Progress in Retinal and Eye Research·Lawrence J RizzoloWei Xiao
Mar 13, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Vanessa ArecoNori Tolosa de Talamoni
Sep 2, 2016·Tissue Barriers·Olga J Baker
Jun 8, 2012·Annals of the New York Academy of Sciences·Susanne M KrugAlan S L Yu
Jun 29, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ozkan OzdenBrenda J Grubb
Oct 6, 2007·Biochimica Et Biophysica Acta·Hideki ChibaNorimasa Sawada
Mar 19, 2013·Inflammatory Bowel Diseases·Marina JuricOliver Bachmann
Oct 28, 2016·American Journal of Physiology. Renal Physiology·Shigeaki Muto
Sep 14, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Tetsuichiro InaiYosaburo Shibata
Jul 5, 2017·Journal of the American Society of Nephrology : JASN·Ernie M H F BongersTom Nijenhuis
Jun 29, 2006·American Journal of Physiology. Renal Physiology·Christina M Van ItallieJames M Anderson
Aug 5, 2017·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Smail Hadj-RabiaHatem El-Shanti
Apr 17, 2013·Physiological Reviews·Dorothee Günzel, Alan S L Yu
Aug 21, 2019·International Journal of Molecular Sciences·Yosuke HashimotoMasuo Kondoh
Jun 22, 2017·Annals of the New York Academy of Sciences·Rita RosenthalMichael Fromm
Jul 2, 2019·Molecular Medicine Reports·Nancy Machorro-RojasMaria Elisa Drago-Serrano
Nov 3, 2015·World Journal of Gastroenterology : WJG·Tyler J CaronSusan J Hagen
Nov 2, 2019·International Journal of Molecular Sciences·Susanne Milatz
Jul 14, 2020·World Journal of Gastroenterology : WJG·Vanessa A ArecoMaría E Peralta López
Jun 26, 2015·World Journal of Gastroenterology : WJG·Gabriela Diaz de BarbozaNori Tolosa de Talamoni
Apr 18, 2017·Annals of the New York Academy of Sciences·Yosuke HashimotoMasuo Kondoh
Dec 13, 2016·Pflügers Archiv : European journal of physiology·Susanne Milatz, Tilman Breiderhoff
Feb 16, 2018·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yosuke HashimotoMasuo Kondoh
Mar 19, 2021·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Rosa Vargas-Poussou
Apr 23, 2009·Journal of Cell Science·Dorothee GünzelDominik Müller
Apr 19, 2021·Cellular and Molecular Gastroenterology and Hepatology·Felix Alexander BaierDeborah Stroka
Jun 22, 2021·American Journal of Physiology. Renal Physiology·Caroline Prot-BertoyeHenrik Dimke
Sep 7, 2021·Orbit·Zhenyang Zhao, Richard C Allen

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