Abstract
Small proteoglycans were dissociatively extracted from normal human thoracic aorta with 4 M guanidine hydrochloride containing protease inhibitors, and purified by Sepharose CL-4B chromatography, dissociative cesium chloride density gradient centrifugation, and diethylaminoethyl cellulose chromatography. The intact proteoglycans migrated in the 270,000-340,000 range on 4-20% sodium dodecyl sulfate polyacrylamide gradient gels. Core proteins prepared following digestion of the intact proteoglycan monomer with chondroitinase ABC consisted of a major Coomassie blue-staining protein band of 50,000 along with a minor band of 44,000. Subsequent studies using endoglycosidases H, F, and N-glycanase demonstrated that mainly complex type N-linked glycans were present on the 50,000 cores while the 44,000 cores appeared to be devoid of N-linked glycans. Western blotting demonstrated that both of these cores were recognized by the monoclonal antibody 2-B-6, indicating the presence of the terminal 4-sulfated unsaturated disaccharide (delta Di-4S) remaining on the linkage region following chondroitinase ABC digestion. In contrast, a diffuse pattern of delta Di-4S epitopes ranging from 50,000 to approximately 60,000 was observed following chon...Continue Reading
References
Aug 1, 1976·The Journal of Clinical Investigation·R L StevensK Schmid
Dec 1, 1976·Clinical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Jan 1, 1977·Journal of Supramolecular Structure·V C Hascall
May 1, 1977·Analytical Biochemistry·K D Curwen, S C Smith
Feb 1, 1978·Atherosclerosis·M TammiM Möttönen
Jul 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·W D WagnerH A Rowe
Apr 1, 1985·The Journal of Cell Biology·L M FritzeR D Rosenberg
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T Krusius, E Ruoslahti
Jan 1, 1986·Annual Review of Biochemistry·J R HassellV C Hascall
Feb 1, 1987·Carbohydrate Research·H Uchiyama, K Nagasawa
Nov 1, 1987·Journal of the Neurological Sciences·A BertolottoB Caterson
May 30, 1986·Journal of Chromatography·J J L'Italien
Jan 1, 1985·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H A Rowe, W D Wagner
Oct 1, 1985·European Journal of Biochemistry·W HoppeH Kresse
Aug 15, 1985·The Biochemical Journal·D HeinegårdK Vogel
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Jan 1, 1974·Advances in Experimental Medicine and Biology·E B Smith
Sep 1, 1970·Analytical Biochemistry·N SenoS Saito
Dec 1, 1968·Analytical Biochemistry·E Wessler
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L A FranssonA Malmström
Nov 1, 1981·Analytical Biochemistry·J H Morrissey
Nov 1, 1983·The Biochemical Journal·J GlösslH Kresse
Nov 1, 1984·The Biochemical Journal·K G VogelD Heinegård
Jul 1, 1981·The Biochemical Journal·I CarlstedtA Malmström
Jan 1, 1982·Advances in Lipid Research·G Camejo
Oct 1, 1980·The Biochemical Journal·Y OikeS Suzuki
Aug 1, 1981·The Biochemical Journal·D HeinegårdC Carlström
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·W D Wagner, S R Nohlgren
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER
Citations
Jan 4, 1993·Atherosclerosis·T C RegisterM O Lively
May 15, 1993·Experientia·H KresseE Schönherr
Feb 17, 1998·Journal of Microencapsulation·T ModenaA Gazzaniga
Mar 31, 1995·The Journal of Biological Chemistry·K SugaharaA Ikegami
May 1, 1994·Circulation·H C StaryR W Wissler
Sep 1, 1995·Circulation·H C StaryR W Wissler
Jan 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·R A RobbinsB Caterson
Jul 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·I J EdwardsW D Wagner
May 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H C StaryR W Wissler
Sep 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·R A ShirkW D Wagner
Jul 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·E SchönherrT N Wight
Sep 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·H C StaryR W Wissler
Jan 11, 1992·Circulation·H C StaryW D Wagner
Jan 11, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·H C StaryW D Wagner
Jan 8, 2000·The Journal of Biological Chemistry·T KajiM G Kinsella