Heterogeneity in Induction Level, Infection Ability, and Morphology of Shiga Toxin-Encoding Phages (Stx Phages) from Dairy and Human Shiga Toxin-Producing Escherichia coli O26:H11 Isolates

Applied and Environmental Microbiology
Ludivine BonannoFrédéric Auvray

Abstract

Shiga toxin (Stx)-producing Escherichia coli (STEC) bacteria are foodborne pathogens responsible for diarrhea and hemolytic-uremic syndrome (HUS). Shiga toxin, the main STEC virulence factor, is encoded by the stx gene located in the genome of a bacteriophage inserted into the bacterial chromosome. The O26:H11 serotype is considered to be the second-most-significant HUS-causing serotype worldwide after O157:H7. STEC O26:H11 bacteria and their stx-negative counterparts have been detected in dairy products. They may convert from the one form to the other by loss or acquisition of Stx phages, potentially confounding food microbiological diagnostic methods based on stx gene detection. Here we investigated the diversity and mobility of Stx phages from human and dairy STEC O26:H11 strains. Evaluation of their rate of in vitro induction, occurring either spontaneously or in the presence of mitomycin C, showed that the Stx2 phages were more inducible overall than Stx1 phages. However, no correlation was found between the Stx phage levels produced and the origin of the strains tested or the phage insertion sites. Morphological analysis by electron microscopy showed that Stx phages from STEC O26:H11 displayed various shapes that were unr...Continue Reading

References

May 1, 1982·Cell·J W Little, D W Mount
Aug 26, 1998·Infection and Immunity·D W AchesonM K Waldor
Nov 1, 2001·Research in Microbiology·H Schmidt
May 16, 2002·Molecular Microbiology·Patrick L WagnerMatthew K Waldor
Oct 21, 2004·International Journal of Medical Microbiology : IJMM·Sylvia HeroldHerbert Schmidt
Jan 18, 2005·Journal of Dairy Science·H S Hussein, T Sakuma
Mar 5, 2005·Applied and Environmental Microbiology·Abram AertsenChris W Michiels
Mar 23, 2005·Lancet·Phillip I TarrWayne L Chandler
Sep 20, 2005·The Journal of Infectious Diseases·John T BrooksNancy A Strockbine
Dec 22, 2005·Foodborne Pathogens and Disease·Mary MurphySéamus Fanning
Apr 3, 2007·Applied and Environmental Microbiology·Martina BielaszewskaHelge Karch
Nov 18, 2008·Applied and Environmental Microbiology·Cristina García-AljaroAnicet R Blanch
Dec 11, 2008·Methods in Molecular Biology·Andrew M KropinskiRoger P Johnson
Jan 27, 2009·Applied and Environmental Microbiology·Lejla ImamovicMaite Muniesa
Sep 25, 2010·Seminars in Thrombosis and Hemostasis·Lothar-Bernd ZimmerhacklHelge Karch
Mar 29, 2011·Applied and Environmental Microbiology·Lejla Imamovic, Maite Muniesa
Apr 17, 2012·Applied and Environmental Microbiology·Maite MuniesaHarald Brüssow
Jul 5, 2012·Journal of Clinical Microbiology·Flemming ScheutzAlison D O'Brien
Sep 4, 2012·International Journal of Food Microbiology·Choreh FarrokhOlivier Cerf
Feb 6, 2014·International Journal of Food Microbiology·M TrevisaniS Bonardi
Jul 8, 2014·Frontiers in Cellular and Infection Microbiology·Rosangela TozzoliStefano Morabito
Dec 3, 2014·Research in Microbiology·Lesley HoylesDouwe van Sinderen

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Citations

Aug 20, 2020·Critical Reviews in Biotechnology·Graça PintoJoana Azeredo
Apr 11, 2020·Frontiers in Microbiology·Rosalind A GilbertEleanor Jameson
Sep 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Brandon M SyJai J Tree
Jul 31, 2021·FEMS Microbiology Ecology·Graça PintoJoana Azeredo

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