Heterogeneity in junctional regions of immunoglobulin kappa deleting element rearrangements in B cell leukemias: a new molecular target for detection of minimal residual disease

Leukemia
A BeishuizenJ J van Dongen

Abstract

Virtually all immunoglobulin kappa (IGK) gene deletions are mediated via rearrangements of the so-called kappa deleting element (Kde). Kde rearrangements occur either to Vkappa gene segments (Vkappa-Kde rearrangements) or to the heptamer recombination signal sequence in the Jkappa-Ckappa intron. Kde rearrangements were analyzed by the polymerase chain reaction (PCR) and heteroduplex analysis in 130 B-lineage leukemias: 63 precursor-B-acute lymphoblastic leukemias (ALL) and 67 chronic B cell leukemias. To obtain detailed information about Kde rearrangements, we sequenced 109 of the 189 detected junctional regions. Vkappa gene family usage in the Vkappa-Kde rearrangements in our series of B-lineage leukemias was comparable to Vkappa gene family usage in functional Vkappa-Jkappa rearrangements in normal and malignant mature B cells, except for a higher frequency of VkappaII family usage in precursor-B-ALL. Junctional region sequencing of the Kde rearrangements in precursor-B-ALL revealed a mean insertion of 4.7 nucleotides and a mean deletion of 9.5 nucleotides, resulting in an extensive junctional diversity, whereas in chronic B cell leukemias the insertion (1.9) and deletion (6.0) were significantly lower. The relatively extensi...Continue Reading

Citations

Apr 29, 2005·The Journal of Molecular Diagnostics : JMD·Reetesh K PaiDavid S Viswanatha
Feb 26, 1999·Leukemia & Lymphoma·F BertoniE Zucca
May 11, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Alessandra SottiniLuisa Imberti
Dec 23, 2003·British Journal of Haematology·Francesco BertoniFinbarr E Cotter
Jun 6, 2003·British Journal of Haematology·Dario Campana
Jun 13, 2013·Cytometry. Part B, Clinical Cytometry·Giuseppe GaipaDario Campana
May 4, 2002·Best Practice & Research. Clinical Haematology·Tomasz SzczepańskiJacques J M van Dongen
May 4, 2002·Best Practice & Research. Clinical Haematology·Faith E DaviesGareth J Morgan
Aug 12, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Federica LovisaAngelo Rosolen
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Menno C van ZelmJacques J M van Dongen
Nov 9, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico SeranaLuisa Imberti
Nov 26, 2002·Hematology·Dieter HoelzerTomasz Szczepański

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.