Heterogeneity in phenotypic and genotypic characteristics among strains of Hafnia alvei.

Journal of Clinical Microbiology
A IsmailiP M Sherman

Abstract

Hafnia alvei is an emerging human pathogen associated with sporadic cases and outbreaks of diarrhea. Bangladeshi isolates of H. alvei possess the Escherichia coli attaching and effacing (eaeA) gene and demonstrate an attaching and effacing phenotype. In the present study we examined 11 Canadian H. alvei isolates and strain 19,982 from Bangladesh to determine if the formation of attaching and effacing lesions is a property shared among multiple isolates. Attaching and effacing lesions were detected by induction of tyrosine kinase protein phosphorylation and cytoskeletal rearrangements in infected tissue culture epithelial cells with immunofluorescence microscopy and by the examination of infected cells with transmission electron microscopy. The presence of the eaeA gene was examined by PCR and colony blot hybridization. Profiles of outer membrane protein extracts, chromosomal macrorestriction fragments, and plasmids were also examined. Accumulation of host phosphotyrosine proteins and rearrangement of the cytoskeletal protein alpha-actinin were both observed in HEp-2 cells infected with H. alvei 19,982. In contrast, none of the other 11 clinical H. alvei isolates demonstrated either of these responses, nor did they form attachin...Continue Reading

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Citations

Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·Geert HuysJean Swings
Jan 19, 2006·Clinical Microbiology Reviews·J Michael Janda, Sharon L Abbott
Jul 16, 1999·Journal of Clinical Microbiology·J M JandaM J Albert
Jun 27, 2020·EcoSal Plus·Tânia A T GomesTetsuya Hayashi
May 31, 2002·Journal of Clinical Microbiology·J Michael Janda, Sharon L Abbott

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