PMID: 15332606Aug 31, 2004Paper

Heterogeneity in proliferative potential of ovine mesenchymal stem cell colonies

Journal of Materials Science. Materials in Medicine
N P RhodesC Longinotti

Abstract

Bone marrow biopsies were taken from the iliac crest of 28 individual sheep from three different breeds, ranging in age from 4 months to 8 years and mesenchymal stem cells (MSCs) isolated using selection due to plastic adherence. Cells were cultured in medium that had been selected for its effect on observed MSC proliferation, until populations of greater than 50 million had been obtained from each biopsy. The identity of the isolated cell populations as progenitors of the mesenchymal lineage was verified by deriving both osteoblastic and chondrocytic phenotypes when cultured in osteogenic and chondrogenic medium supplements, respectively. The rate of cell proliferation for each marrow biopsy was measured at each passage and the number of initial stem cells in each sample estimated. There was no statistically significant correlation between the age of the sheep and MSC proliferative potential, or age and estimated initial MSC number. There was no apparent significant difference between proliferation rate and sheep breed and colonies established from frozen cells grew at similar rates to pre-frozen cells. Counter intuitively, there appeared to be a negatively correlated trend between proliferation rate and MSC concentration in t...Continue Reading

Citations

Sep 25, 2012·BMC Veterinary Research·Jaber LyahyaiInmaculada Martín-Burriel
Apr 6, 2011·PloS One·Erika KužmováGeorge A Bubenik
Feb 28, 2007·Clinical Laboratory·Andreas PanskyEdda Tobiasch
Apr 7, 2009·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Joanne RaghunathAlexander M Seifalian
Dec 31, 2008·Journal of Cellular Physiology·Rosa C McCartyCory J Xian
Jul 19, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Etienne J O O'BrienCyril B Frank
Mar 9, 2006·Izvestiia Akademii nauk. Seriia biologicheskaia·O V PaniushinV I Starostin
Nov 2, 2019·International Journal of Molecular Sciences·Heba AbdelrazikAlessandra Pelagalli
Apr 4, 2021·Biology·Rui Damásio AlvitesAna Colette Maurício
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Ejaz R DarTalha Bin Emran

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