Heterogeneity in the ability of IgG1 monoclonal anti-D to promote lymphocyte-mediated red cell lysis.

European Journal of Immunology
B M KumpelB A Bradley

Abstract

Thirty-four IgG anti-D human monoclonal antibodies (mAb) derived from 18 donor were assessed for their ability to mediate lysis of D+ red cells by lymphocytes in antibody-dependent cell-mediated cytotoxicity assays. Cell-bound antibody was quantified and the mAb were compared at similar levels of sensitization. The majority (23/31) of IgG1 and all (3/3) IgG3 mAb were ineffective; two donors produced both lytic and non-lytic anti-D mAb. Greater sensitivity was achieved using fluid-phase antibody (as culture supernatants) in the assay than was obtained with pre-sensitized red cells. Minimum levels of 2000 anti-D molecules per cell were required for lysis using pre-sensitized cells. Partial D red cells (DIVa, DVa and DVI) were lysed by three mAb that were lytic with normal D+ cells. There was no relationship between lytic ability and Gm allotype or D epitope specificity of the antibodies. Four mAb to other blood group specificities were tested: two (anti-E and anti-G) were lytic and two (anti-c and anti-Kell) were not lytic. Possible reasons for the heterogeneity of the lytic activity by the mAb are discussed.

References

Apr 25, 1977·Biochemical and Biophysical Research Communications·N KoideT Muramatsu
Jan 1, 1989·Clinical and Laboratory Haematology·A G HadleyB Zupanska
Sep 1, 1988·Transfusion Medicine Reviews·J M Bowman
Apr 1, 1988·Revue française de transfusion et immuno-hématologie·B M KumpelP A Judson
Feb 19, 1983·Lancet·D H CrawfordE R Huehns
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Nose, H Wigzell
Sep 1, 1986·Immunology Today·C L Anderson, R J Loone

❮ Previous
Next ❯

Citations

Mar 14, 2002·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·Belinda M Kumpel
Sep 24, 1998·Vox Sanguinis·A BlancherP Bénard
Nov 1, 1995·British Journal of Haematology·A G HadleyR J Sokol
Aug 1, 1990·Journal of Immunogenetics·B M Kumpel
Sep 10, 2002·Transplant Immunology·Belinda M Kumpel
Jun 1, 1993·Baillière's Clinical Haematology·A G Hadley, B M Kumpel
Oct 1, 1995·Transfusion Medicine Reviews·A Fletcher, A Thomson
Apr 4, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·R Béliard
May 27, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Leif Kofoed NielsenMorten Hanefeld Dziegiel
Feb 1, 1993·European Journal of Immunology·N YousafB D Williams

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.