PMID: 9435069Jan 22, 1998Paper

Heterogeneity in the attachment and uptake mechanisms of the Legionnaires' disease bacterium, Legionella pneumophila, by protozoan hosts

Applied and Environmental Microbiology
O S HarbYousef Abu Kwaik

Abstract

Invasion and intracellular replication of Legionella pneumophila within protozoa in the environment plays a major role in the transmission of Legionnaires' disease. Intracellular replication of L. pneumophila within protozoa occurs in a rough endoplasmic reticulum (RER)-surrounded phagosome (Y. Abu Kwaik, Appl. Environ. Microbiol. 62:2022-2028, 1996). Since the subsequent fate of many intracellular pathogens is determined by the route of entry, we compared the mechanisms of attachment and subsequent uptake of L. pneumophila by the two protozoa Hartmannella vermiformis and Acanthamoeba polyphaga. Our data provide biochemical and genetic evidence that the mechanisms of attachment and subsequent uptake of L. pneumophila by the two protozoan hosts are, in part, different. First, uptake of L. pneumophila by H. vermiformis is completely blocked by the monovalent sugars galactose and N-acetyl-D-galactosamine, but these sugars partially blocked A. polyphaga. Second, attachment of L. pneumophila to H. vermiformis is associated with a time-dependent and reversible tyrosine dephosphorylation of multiple host proteins. In contrast, only a slight dephosphorylation of a 170-kDa protein of A. polyphaga is detected upon infection. Third, synth...Continue Reading

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Citations

Jun 10, 2000·Microbiology·S L CirilloJ D Cirillo
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Jan 11, 2005·Applied and Environmental Microbiology·Maëlle MolmeretYousef Abu Kwaik
Jul 16, 2008·Applied and Environmental Microbiology·Maria A SöderbergNicholas P Cianciotto
Jun 19, 2002·FEMS Microbiology Reviews·Michael SteinertJörg Hacker
May 26, 1999·Critical Reviews in Microbiology·D CorsaroM Valassina
Mar 24, 2009·Journal of Applied Microbiology·H Y Lau, N J Ashbolt
Aug 30, 2020·FEMS Microbiology Letters·Rafik DeyPierre Pernin
Dec 7, 2006·FEMS Microbiology Letters·Emily C RobertsEmma C Wootton
Mar 22, 2014·Parasitology Research·Gustavo MedinaHeriberto Fernandez
Dec 19, 2017·Frontiers in Cellular and Infection Microbiology·David K BoamahTamara J O'Connor
Sep 3, 1998·Applied and Environmental Microbiology·Y Abu KwaikO S Harb
Nov 2, 2018·Nature Communications·Tomonori KanekoShawn S-C Li
Oct 19, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Elif Özlem Arslan-Aydoğdu, Ayten Kimiran
Feb 27, 1999·Current Opinion in Microbiology·J P Vogel, R R Isberg

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