Heterogeneity in the phosphorylation of human death receptors by p42(mapk/erk2)

Biochemical and Biophysical Research Communications
S K FrankelD W Riches

Abstract

Phosphorylation of murine CD120a by p42(mapk/erk2) has been shown to inhibit its ability to initiate apoptosis while preserving signaling events such as NF-kappaB activation. Therefore, we sought to determine if p42(mapk/erk2) was also capable of phosphorylating additional human death receptors within the TNF receptor superfamily. These studies showed that CD120a and DR3 are significantly phosphorylated by p42(mapk/erk2) but Fas, DR4 and DR5 are not. Additionally, we demonstrated that (i) the p42(mapk/erk2)-dependent phosphorylation of CD120a and DR3 occurred on Ser and Thr residues, (ii) p42(mapk/erk2) phosphorylated residues located in the membrane proximal regions but not the death domains of CD120a and DR3, (iii) Ser 253 is a preferred site of phosphorylation on CD120a, and (iv) the p42(mapk/erk2)-dependent phosphorylation of the DR3 cytoplasmic domain occurred exclusively at non-p42/44(mapk/erk2/1) consensus sites. These findings suggest that human death receptors segregate into two groups along lines of phylogeny with respect to Ser/Thr phosphorylation by p42(mapk/erk2).

References

Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·E GoillotI Sanchez
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Aug 26, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·A SinghB B Aggarwal
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Jun 8, 1999·Cellular Immunology·D J WilsonR C Budd
Jun 8, 1999·Annual Review of Immunology·D WallachM P Boldin
Mar 10, 2001·Nature·L Chang, M Karin

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Citations

Apr 21, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Narasaiah Kolliputi, Aaron B Waxman
Nov 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Stephen K FrankelDavid W H Riches
Feb 25, 2005·Neurosurgical Focus·Bimal G RamiSatyendra K Singh
Dec 8, 2009·Biochimica Et Biophysica Acta·Bodvaël PennarunSteven de Jong
Mar 26, 2003·Cell Death and Differentiation·J T BridghamA L Johnson
Apr 30, 2004·American Journal of Physiology. Cell Physiology·Laurie E KilpatrickHelen M Korchak
Feb 10, 2004·The Journal of Biological Chemistry·Shyra J GardaiPeter M Henson
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas S SöderströmJohn E Eriksson

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