PMID: 6411469Sep 1, 1983Paper

Heterogeneity of brush-border-membrane vesicles from rat small intestine prepared by a precipitation method using Mg/EGTA

European Journal of Biochemistry
Bruno Stieger, H Murer

Abstract

Brush border membrane vesicles from rat small intestine were isolated by a Mg/EGTA precipitation method. Further fractionation either by free flow electrophoresis or by sucrose density gradient centrifugation leads to subfractions which differ with respect to enzyme enrichment factors, transport properties for D-glucose and protein pattern analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. A relative enrichment of (Na+ + K+)-ATPase is found in one fraction, whereas in another fraction maltase, aminopeptidase M and alkaline phosphatase are relatively enriched. The fractions show different properties of D-glucose transport under tracer exchange conditions and a different inhibition of D-glucose transport by phlorizin and phloretin. These results indicate that the vesicles obtained from rat small intestine by this cation precipitation method are not homogeneous. The inhomogeneity cannot be due to a crosscontamination by membranes other than from the cell envelopment, as none of the fractions show a significant enrichment of succinate--cytochrome c oxidoreductase, KCN-resistant NADH oxidoreductase or glucosaminidase. The inhomogeneity might be due either to a crosscontamination by basal-lateral membranes or to m...Continue Reading

References

Apr 15, 1978·The Biochemical Journal·W HaaseR Kinne
Feb 24, 1976·Pflügers Archiv : European journal of physiology·W Berner, R Kinne
Sep 27, 1973·Biochimica Et Biophysica Acta·J SchmitzR K Crane
Jan 1, 1982·Physiological Reviews·A J Kenny, S Maroux
Nov 18, 1980·Biochimica Et Biophysica Acta·H HauserD E Bowyer
Jan 1, 1983·The Journal of Membrane Biology·B StiegerH Murer
Jul 15, 1980·The Journal of Membrane Biology·H Murer, R Kinne
Jan 1, 1981·The International Journal of Biochemistry·K Y LingR G Faust

❮ Previous
Next ❯

Citations

Mar 1, 1995·Medicinal Research Reviews·K M HillgrenR T Borchardt
Jan 1, 1994·Pflügers Archiv : European journal of physiology·M LeviJ Biber
Sep 1, 1989·Pflügers Archiv : European journal of physiology·C R DunkL A Turnberg
Feb 9, 2011·European Journal of Drug Metabolism and Pharmacokinetics·Yusuke TakizawaMasahiro Hayashi
Sep 1, 1994·Comparative Biochemistry and Physiology. Physiology·S WolfframE Scharrer
Sep 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S A Ibrahim, K A Balasubramanian
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·T VerriC Storelli
Jun 5, 2007·Nature Protocols·Jürg BiberHeini Murer
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·H HilfikerJ Biber
Feb 24, 2001·British Poultry Science·D KingO Adeola
Nov 10, 2009·Pharmacogenetics and Genomics·Yoshihiro KonnoMasahiko Negishi
Jan 1, 1994·The Journal of Pharmacy and Pharmacology·P LangguthH P Merkle
Apr 1, 1991·Gut·A J WatsonP D Fairclough
Mar 5, 2011·Biological & Pharmaceutical Bulletin·Yusuke TakizawaMasahiro Hayashi
Nov 1, 2005·Environmental Toxicology and Pharmacology·D S GomesE C A Eleutherio
Apr 14, 1995·Mechanisms of Ageing and Development·L TeilletB Corman
Dec 1, 1987·Mechanisms of Ageing and Development·A D Mooradian, M K Song
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J F GregoryJ J Cerda
Sep 5, 2002·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Juan CondominaAna Polache
Aug 1, 1986·Kidney International·H Murer, P Gmaj
Sep 25, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shinji SakumaZheng-Rong Lu
Jul 31, 1990·Biochemical and Biophysical Research Communications·D J Bjorkman, E J Brigham
May 1, 1991·JPEN. Journal of Parenteral and Enteral Nutrition·S A Acra, F K Ghishan
Oct 31, 2006·Drug Metabolism and Pharmacokinetics·Aiko IidaMasahiro Hayashi
Feb 11, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tamara RadanovicJürg Biber
Mar 3, 2020·Journal of Nutritional Science and Vitaminology·Yasuhiro IchidaNaoshi Horiba
Nov 1, 2008·Drug Metabolism and Pharmacokinetics·Mikio TomitaMasahiro Hayashi
Jun 19, 2001·Biochimica Et Biophysica Acta·M C De la HorraA A Ilundáin
Jun 11, 1985·Biochimica Et Biophysica Acta·M W RiglerJ S Patton
Nov 13, 1991·Biochimica Et Biophysica Acta·P Proulx

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.