Heterogeneity of dynein structure implies coordinated suppression of dynein motor activity in the axoneme

Journal of Structural Biology
Aditi Maheshwari, Takashi Ishikawa

Abstract

Axonemal dyneins provide the driving force for flagellar/ciliary bending. Nucleotide-induced conformational changes of flagellar dynein have been found both in vitro and in situ by electron microscopy, and in situ studies demonstrated the coexistence of at least two conformations in axonemes in the presence of nucleotides (the apo and the nucleotide-bound forms). The distribution of the two forms suggested cooperativity between adjacent dyneins on axonemal microtubule doublets. Although the mechanism of such cooperativity is unknown it might be related to the mechanism of bending. To explore the mechanism by which structural heterogeneity of axonemal dyneins is induced by nucleotides, we used cilia from Tetrahymena thermophila to examine the structure of dyneins in a) the intact axoneme and b) microtubule doublets separated from the axoneme, both with and without additional pure microtubules. We also employed an ATPase assay on these specimens to investigate dynein activity functionally. Dyneins on separated doublets show more activation by nucleotides than those in the intact axoneme, both structurally and in the ATPase assay, and this is especially pronounced when the doublets are coupled with added microtubules, as expected....Continue Reading

References

Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·K A Johnson
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·K E Summers, I R Gibbons
Aug 1, 1980·The Journal of Cell Biology·F D Warner, N C Zanetti
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
Jul 27, 2001·Journal of Structural Biology·J B Heymann
Mar 1, 2003·Nature·Stan A BurgessKazuhiro Oiwa
Aug 19, 2003·The Journal of Biological Chemistry·Miho Sakato, Stephen M King
Dec 3, 2003·Cell Motility and the Cytoskeleton·Elizabeth F Smith, Pinfen Yang
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoshinori TakahashiYoko Y Toyoshima
Mar 30, 2007·Journal of Molecular Biology·Takashi IshikawaKazuhiro Oiwa
Apr 18, 2007·The Journal of Cell Biology·Toshiyuki OdaMasahide Kikkawa
Jul 17, 2007·Cell Motility and the Cytoskeleton·Yuichi Inoue, Chikako Shingyoji
Nov 26, 2008·The Journal of Cell Biology·Khanh Huy BuiTakashi Ishikawa
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hironori UenoKeiko Hirose
Dec 17, 2008·Science·Andrew P CarterI R Gibbons
Feb 11, 2009·Cell·Anthony J RobertsStan A Burgess
Aug 12, 2009·The Journal of Cell Biology·Khanh Huy BuiTakashi Ishikawa
May 11, 2010·Nature Structural & Molecular Biology·Tandis MovassaghTakashi Ishikawa
Feb 19, 2011·Science·Andrew P CarterRonald D Vale
May 24, 2011·Nature Structural & Molecular Biology·Takahide KonGenji Kurisu
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniela NicastroRichard W Linck
Jun 6, 2012·Journal of Structural Biology·Takashi Ishikawa

❮ Previous
Next ❯

Citations

Mar 5, 2014·The Journal of Cell Biology·Toshiyuki OdaMasahide Kikkawa
Feb 19, 2015·Biophysical Journal·Shin YamaguchiJunichiro Yajima
Sep 16, 2015·Microscopy·Shiori TobaShinji Hirotsune
Jun 6, 2012·Journal of Structural Biology·Takashi Ishikawa
Oct 7, 2014·Biochemical and Biophysical Research Communications·Masaki Edamatsu
Sep 8, 2016·Cold Spring Harbor Perspectives in Biology·Takashi Ishikawa
Oct 16, 2012·Structure·Carolyn A Moores

❮ Previous
Next ❯

Related Concepts