Abstract
Entamoeba histolytica (Eh), the parasite that causes amebic dysentery, is the only protozoan that phagocytoses bacteria, epithelial cells and red blood cells. Numerous low-molecular weight GTP-binding proteins, called p21rac, are implicated in signal transduction and actin polymerization during phagocytosis by macrophages and Dictyostelium discoideum (Dd). Here, molecular cloning techniques were used to obtain four Eh rac genes that encoded putative p21rac, as well as segments of two Eh rac pseudogenes. The predicted Eh p21rac, which share 55-81% amino acid (aa) identities with each other, include one that closely resembles the p21rac1 of man, Dd, Drosophila melanogaster and Caenorhabditis elegans; two that resemble the p21racC of Dd; and one that is unique. An alignment of the Eh rac ORF with other rac family proteins reveals multiple aa that distinguish p21rac1, p21racC and p21cdc42. We conclude that the Eh genes encoding amebic p21rac, which are the first identified from a protozoan parasite, are numerous and heterogeneous.
References
Jan 1, 1992·Protein Engineering·R F Smith, T F Smith
Aug 7, 1992·Cell·A J Ridley, A Hall
Aug 7, 1992·Cell·A J RidleyA Hall
Jul 1, 1991·Molecular and Cellular Biology·M ZimanD I Johnson
May 14, 1991·Biochemistry·A ValenciaC Sander
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K ShinjoR A Cerione
Jul 1, 1990·The Journal of Cell Biology·A E AdamsJ R Pringle
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 1, 1985·The Journal of Experimental Medicine·G B BaileyJ W Gasque
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P MadauleA M Myers
Apr 7, 1995·Cell·C D Nobes, A Hall
May 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D RobertsonP Monaghan
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J I Ravdin
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Aug 1, 1994·Genes & Development·L LuoY N Jan
Jul 22, 1994·Science·D DiekmannA Hall
Mar 1, 1994·Molecular and Biochemical Parasitology·P S ShenJ Samuelson
Dec 22, 1993·Gene·J BushJ Cardelli
Mar 1, 1993·Molecular and Biochemical Parasitology·A Lohia, J Samuelson
Citations
May 10, 2000·Molecular and Biochemical Parasitology·G D Godbold, B J Mann
May 30, 2002·Molecular and Biochemical Parasitology·Brenda H WelterLesly A Temesvari
Sep 23, 1997·Molecular and Biochemical Parasitology·S S GangopadhyayA Lohia
Mar 13, 1998·Molecular and Biochemical Parasitology·S S GangopadhyayA Lohia
Nov 7, 1999·Molecular and Biochemical Parasitology·L A TemesvariJ A Cardelli
Aug 26, 1999·Parasitology Today·M C FieldH Field
Oct 12, 2012·Biochemistry·Dustin E BoschDavid P Siderovski
Mar 23, 2013·Experimental & Molecular Medicine·Dustin E Bosch, David P Siderovski
Sep 21, 2011·The Journal of Biological Chemistry·Dustin E BoschDavid P Siderovski
Feb 27, 2001·Nucleic Acids Research·F RiveroA A Noegel
Oct 24, 2006·Applied and Environmental Microbiology·Shubhra MajumderKlaus Aktories
Feb 17, 2005·Experimental Parasitology·Arturo Aguilar-RojasMiguel Vargas
Sep 24, 2004·Biochemical and Biophysical Research Communications·Luiz Dione B de MeloUlisses Gazos Lopes
Jul 14, 2004·Biochemical and Biophysical Research Communications·Gursharan Kaur, Anuradha Lohia
Nov 23, 2005·Molecular and Biochemical Parasitology·Asao MakiokaTomoyoshi Nozaki
Jun 16, 2005·Experimental Parasitology·Yumiko Saito-NakanoTomoyoshi Nozaki
Jun 6, 2009·Cellular Microbiology·Kumiko Nakada-TsukuiTomoyoshi Nozaki
Jun 27, 2007·Bioscience Reports·Anuradha LohiaPromita Ghosh Dastidar
Aug 25, 1999·Current Opinion in Microbiology·C A Gilchrist, W A Petri
Oct 20, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G D Godbold, B J Mann
May 24, 2006·Molecular and Biochemical Parasitology·Luis Enrique Arias-RomeroMiguel Vargas
May 10, 2017·Cellular Microbiology·Ravi BharadwajAlok Bhattacharya
Oct 30, 2003·The Journal of Biological Chemistry·Masahiro KumagaiTomoyoshi Nozaki
May 25, 1999·Infection and Immunity·S K GhoshJ Samuelson
Oct 8, 1997·Infection and Immunity·S K Ghosh, J Samuelson
Jul 3, 2021·Cells·Vedrana FilićIgor Weber
Dec 5, 2000·Genetics·P WingeA M Bones