Heterogeneity of growth hormone (GH)-producing cells in aging male rats: in vitro GH releasing activity of somatotrope subpopulations

Molecular and Cellular Endocrinology
P M Dobado-BerriosF Gracia-Navarro

Abstract

Studies on the age-related decline of growth hormone (GH) release have ignored that the population of GH-producing cells (somatotropes) is heterogeneous. In aging male rats, centrifugation of dispersed pituitary cells in a density gradient yields two somatotrope subpopulations, i.e. low- (LD) and high-density (HD) cells. A previous analysis of ultrastructure and GH mRNA levels has shown that storage and biosynthetic features were inversely related in both subsets. Furthermore, ultrastructural and molecular differences between LD- and HD-cells were retained throughout the rat lifespan, suggesting that the heterogeneity of somatotropes may have a biological meaning. Accordingly, the main objective of the present study was to analyze the functional heterogeneity of the somatotrope population during the aging process in male rats. For this purpose, the response of LD- and HD-somatotropes from 5-, 19-, and 26-month-old male rats was analyzed with an optimized cell immunoblot assay both under basal conditions, and after GH-releasing factor (GRF) and/or somatostatin (SS) treatments. Simultaneous measurements of hormonal release, intracellular GH content, and cell size were performed at the single-somatotrope level. Average values for ...Continue Reading

References

Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P M Dobado-BerriosF Gracia-Navarro
Mar 1, 1992·The Journal of Endocrinology·M KazemzadehE L Hooghe-Peters
Jan 1, 1991·Molecular Endocrinology·K ScarbroughP M Wise
Dec 1, 1990·Endocrinologia Japonica·S TakahashiN Matsui
Jan 1, 1987·Experimental Gerontology·R G GoyaJ Meites
Mar 1, 1988·Cell and Tissue Research·S MalamedC G Scanes
Aug 1, 1986·Endocrine Reviews·R M SapolskyB S McEwen
Aug 1, 1987·Neuroendocrinology·S TakahashiJ Meites
Nov 1, 1973·The Journal of Cell Biology·C R Hopkins, M G Farquhar
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Dec 1, 1980·Endocrinology·W E SonntagJ Meites
Jul 1, 1994·Neurochemistry International·V De Gennaro ColonnaE E Müller
Aug 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J WatanabeS Kanamura
Feb 1, 1994·Biology of Reproduction·G H Larson, P M Wise
Jun 1, 1993·Journal of Neuroendocrinology·R TorronterasF Gracia-Navarro

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Citations

Jun 18, 1998·Annals of the New York Academy of Sciences·M M MalagónF Gracia-Navarro
Mar 5, 2019·Endocrine Connections·Marko StojanovicMarta Korbonits
Jul 11, 2009·Endocrinology·Francisca Rodríguez-PachecoMaria M Malagón
Sep 19, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Adrian FloreaMarius Cristian Oprea
Jul 24, 2003·American Journal of Physiology. Endocrinology and Metabolism·Rafael Vazquez-MartinezMaria M Malagon
May 13, 2021·Nature Communications·Frederique Ruf-ZamojskiStuart C Sealfon

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