Heterogeneity of human mast cells and basophils. Effects of a putative 5-lipoxygenase inhibitor.

Biochemical Pharmacology
V CohanL M Lichtenstein

Abstract

The effects of a putatively specific 5-lipoxygenase inhibitor, 2(12-hydroxydodeca-5,10-dinyl)-3,5,6-trimethyl-1,4-benzoquin one (AA-861), and its major metabolite, M-I, were assessed using anti-IgE activated human basophils, lung mast cells and skin mast cells. In basophils and lung mast cells, no effects on histamine release were observed, whereas leukotriene C4 (LTC4) production was inhibited (IC50 values less than 1 microM). In addition, prostaglandin D2 (PGD2) production was inhibited in lung mast cells (IC50 congruent to 5 microM). In contrast, in skin mast cells both histamine release and PGD2 production were reduced by AA-861 and M-I, with IC50 values of congruent to 5 and 0.4 microM for histamine and PGD2, respectively. These data reveal biochemical heterogeneity among human histamine-containing cells and underscore the necessity of assessing a pharmacologic agonist in relevant cell systems.

References

Jan 1, 1985·International Archives of Allergy and Applied Immunology·Y MoritaT Miyamoto
Jun 1, 1988·Journal of Clinical Pharmacology·D E Furst
Dec 1, 1983·Prostaglandins·Y AshidaY Maki
Jul 30, 1981·Nature·S P PetersL M Lichtenstein
Nov 25, 1983·Journal of Immunological Methods·S P PetersL M Lichtenstein
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R M ClancyT E Hugli
Oct 1, 1983·Prostaglandins, Leukotrienes, and Medicine·K OhuchiL Levine

❮ Previous
Next ❯

Citations

Jun 1, 1996·In Vitro Cellular & Developmental Biology. Animal·N MatsuoM Sugano
Sep 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y OkayamaM K Church
Aug 17, 2000·Bioscience, Biotechnology, and Biochemistry·N MatsuoM Sugano
Jan 4, 2006·American Journal of Obstetrics and Gynecology·Robert E GarfieldRoberto Romero
Aug 2, 2002·Pediatric Annals·James N Jarvis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.