PMID: 6163134Jan 10, 1981Paper

Heterogeneity of interferon mRNA species from Sendai virus-induced human lymphoblastoid (Namalva) cells and Newcastle disease virus-induced murine fibroblastoid (L) cells

Nucleic Acids Research
A D SagarP B Sehgal

Abstract

Cytoplasmic polyadenylated RNA preparations obtained from Sendai-induced human lymphoblastoid (Namalva) cells and from Newcastle disease virus (NDV)-induced murine (L) cells were denatured in 10-12.5 mM CH3HgOH and then electrophoresed in 2% agarose tube gels containing 10 mM CH3HgOH, the RNA eluted from gel slices and translationally active interferon mRNA species located using the Xenopus oocyte assay. The interferons synthesized were characterized as alpha or beta types based on neutralization tests using specific antisera against human or murine interferon-alpha and interferon-beta. At least two species of mRNA for human interferon-alpha and two for human interferon-beta were detected in RNA from Sendai-induced Namalva cells. These are (approximate mRNA length in parentheses) alpha (1.3 kb), alpha (1.9 kb), beta (1.1 kb) and beta (1.9 kb). Two populations of murine interferon mRNA of lengths approximately 1.4 kb and 3 kb were detected in mRNA preparations from NDV-induced L cells by electrophoresis. However, since the translation products of each of these two populations of mRNA consist of both murine interferon-alpha and murine interferon-beta it is likely that both the 1.4 kb and 3 kb populations contain at least one spec...Continue Reading

References

Jan 1, 1978·The Journal of General Virology·E A HavellJ Vilcek
Jul 1, 1979·The Journal of General Virology·J MorserD C Burke
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L CavalieriS Pestka
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Dec 1, 1972·Antimicrobial Agents and Chemotherapy·E A Havell, J Vilcek

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Citations

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalM Inouye
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalM Inouye
Sep 1, 1982·The Journal of Experimental Medicine·A D SagarF H Ruddle
Jan 1, 1983·Journal of Interferon Research·W P Vonk, J Trapman
Jun 15, 1983·European Journal of Biochemistry·J ShuttleworthD C Burke
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Edge, R Camble
Aug 1, 1982·Archives of Biochemistry and Biophysics·F H SarkarL A Pickering
Jun 11, 1981·Nature·H K HochkeppelJ Collins
Jul 25, 1983·FEBS Letters·O K SharmaB B Goswami
Sep 30, 1982·Biochimica Et Biophysica Acta·P B Sehgal
Nov 13, 1981·Science·P B SehgalI A Braude
Mar 23, 1984·Science·A D SagarF H Ruddle

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