Heterogeneity of intracellular polymer storage states in enhanced biological phosphorus removal (EBPR)--observation and modeling

Environmental Science & Technology
Vanni BucciApril Z Gu

Abstract

A number of agent-based models (ABMs) for biological wastewater treatment processes have been developed, but their skill in predicting heterogeneity of intracellular storage states has not been tested against observations due to the lack of analytical methods for measuring single-cell intracellular properties. Further, several mechanisms can produce and maintain heterogeneity (e.g., different histories, uneven division) and their relative importance has not been explored. This article presents an ABM for the enhanced biological phosphorus removal (EBPR) treatment process that resolves heterogeneity in three intracellular polymer storage compounds (i.e., polyphosphate, polyhydroxybutyrate, and glycogen) in three functional microbial populations (i.e., polyphosphate-accumulating, glycogen-accumulating, and ordinary heterotrophic organisms). Model predicted distributions were compared to those based on single-cell estimates obtained using a Raman microscopy method for a laboratory-scale sequencing batch reactor (SBR) system. The model can reproduce many features of the observed heterogeneity. Two methods for introducing heterogeneity were evaluated. First, biological variability in individual cell behavior was simulated by randomi...Continue Reading

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Citations

May 15, 2013·Applied and Environmental Microbiology·Neil D FredrickFerdi L Hellweger
Apr 15, 2014·Journal of Molecular Biology·Vanni Bucci, Joao B Xavier
Jul 23, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Annalisa Onnis-HaydenApril Z Gu
Jul 28, 2019·Scientific Reports·Junqin YaoYinguang Chen

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