Heterogeneity of Leishmania donovani parasites complicates diagnosis of visceral leishmaniasis: comparison of different serological tests in three endemic regions

PloS One
Elfadil M AbassUlrich Steinhoff

Abstract

Diagnostic tests for visceral leishmaniasis that are based on antigens of a single Leishmania strain can have low diagnostic performance in regions where heterologous parasites predominate. The aim of this study was to investigate and compare the performance of five serological tests, based on different Leishmania antigens, in three endemic countries for visceral leishmaniasis. A total number of 231 sera of symptomatic and asymptomatic cases and controls from three endemic regions of visceral leishmaniasis in East Sudan, North India and South France were evaluated by following serological tests: rKLO8- and rK39 ELISA, DAT (ITMA-DAT) and two rapid tests of rK39 (IT LEISH) and rKE16 (Signal-KA). Overall, rKLO8- and rK39 ELISA were most sensitive in immunocompetent patients from all endemic regions (96-100%) and the sensitivity was reduced to 81.8% in HIV co-infected patients from France. Sera of patients from India demonstrated significantly higher antibody responses to rKLO8 and rK39 compared with sera from Sudan (p<0.0001) and France (p<0.0037). Further, some Indian and Sudanese patients reacted better with rKLO8 than rK39. Sensitivity of DAT (ITMA-DAT) was high in Sudan (94%) and India (92.3%) but low in France being 88.5% and...Continue Reading

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Citations

Jan 17, 2016·Journal of Proteomics·Carlos Sánchez-OvejeroRaúl Manzano-Román
Mar 21, 2017·Dental Clinics of North America·Parish P SedghizadehCarl M Allen
Nov 24, 2016·Acta Tropica·Lily P Martínez AbadHenrique C Teixeira
Jun 6, 2018·The American Journal of Tropical Medicine and Hygiene·Ryan LammSarah-Blythe Ballard
May 8, 2020·Parasite Immunology·Awanish KumarMukesh Samant
Aug 29, 2019·BMC Infectious Diseases·Giovanny HerreraJuan David Ramirez
Sep 13, 2019·BMC Research Notes·Nouh Saad MohamedArwa H Elaagip
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Jan 9, 2019·Frontiers in Cellular and Infection Microbiology·Tegwen MarlaisMichael A Miles
Apr 5, 2020·Scientific Data·Giovanny HerreraJuan David Ramírez
Oct 20, 2020·Parasitology·Joyce Carvalho PereiraVanete Thomaz Soccol
Oct 28, 2020·Microorganisms·Juliana Quero ReimãoAdriano Cappellazzo Coelho
Mar 12, 2021·Frontiers in Cellular and Infection Microbiology·Srija MoulikMitali Chatterjee
Sep 8, 2021·Microbial Genomics·Luz H PatinoJuan David Ramírez

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Methods Mentioned

BETA
serum
ELISA
PCR

Software Mentioned

GraphPad Prism

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