PMID: 697758Jul 15, 1978Paper

Heterogeneity of lysosomes originating from rat liver parenchymal cells. Metabolic relationship of subpopulations separated by density-gradient centrifugation

The Biochemical Journal
H PertoftM Höök

Abstract

1. A crude lysosomal fraction obtained by differential centrifugation of a rat liver homogenate was subjected to zonal centrifugation in iso-osmotic self-generating gradients composed of modified colloidal silica (Percoll). Analysis of relevant marker-enzyme activities shows a continuous band of considerably purified lysosomal particles in the density range 1.04--1.11 g/ml. 2. A relationship between age and buoyant density of the parenchymal lysosomal subpopulations is indicated by the distribution of 125I-labelled asialoglycoproteins in the heterogeneous lysosomes during the catabolism of the glycoprotein. The labelled asialoglycoprotein first appeared in lysosomal particles of low density, which with time progressively acquired a higher density. Furthermore, 30 min after administration the 125I-labelled asialocaeruloplasmin recovered in the light lysosomes was less degraded than the material recovered in the heavy lysosomes. 3. A lysosomal enzyme (arylsulphatase) was found to possess considerably higher isoelectric points in the heavy lysosomes than in the light lysosomes, which is consistent with a relationship between age and density of the lysosomes.

Citations

Mar 16, 2016·The Journal of Cell Biology·Danielle E JohnsonSergio Grinstein
Dec 1, 1983·Toxicology and Applied Pharmacology·H SuzukiT Ono
Jun 1, 1987·Clinical Endocrinology·M YoshinariM Fujishima
May 1, 1990·Journal of Gastroenterology and Hepatology·Y Uchiyama
Dec 1, 1981·Histochemistry·Y UchiyamaH von Mayersbach
Apr 29, 1982·Biochemical and Biophysical Research Communications·D M Chuang
May 1, 1985·Archives of Biochemistry and Biophysics·J M Armstrong, J D Newman
Aug 1, 1987·Analytical Biochemistry·L J Symons, A J Jonas
Sep 1, 1982·Journal of Cellular Physiology·W K Miskimins, N Shimizu
Dec 1, 1990·Journal of Neurochemistry·A Sweet-CorderoR H Scheller
Jul 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H AbrahamssonH Pertoft
Dec 5, 2018·Nature Nanotechnology·KaHo LeungYamuna Krishnan
Apr 25, 2008·Current Protocols in Immunology·Fernando Aniento, Jean Gruenberg
Apr 23, 2008·Current Protocols in Protein Science·Fernando Aniento, Jean Gruenberg
Oct 1, 1991·The Histochemical Journal·G Bou-GhariosI Olsen
Sep 1, 1984·International Journal of Peptide and Protein Research·M Dyson, J M Walker
Sep 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J Stockert, A G Morell
Jul 11, 1983·The American Journal of Physiology·C A SurmaczG E Mortimore
Jul 13, 2000·Journal of Biochemical and Biophysical Methods·H Pertoft
May 21, 1982·Biochimica Et Biophysica Acta·R Hjorth, H Pertoft
Apr 29, 1982·Biochimica Et Biophysica Acta·A BlekenT Berg
Oct 8, 1982·Biochimica Et Biophysica Acta·W W Chen, G L Decker
Jan 1, 1988·The International Journal of Biochemistry·D AbrahamB Winchester
Mar 1, 1986·Journal of Steroid Biochemistry·H KaráskováO Táborský
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K KobayashiS Horiuchi
Dec 12, 1991·Biochimica Et Biophysica Acta·R L Pisoni, J G Thoene
May 1, 1981·Archives of Biochemistry and Biophysics·W W ChenH W Moser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
K KobayashiS Horiuchi
Biochemistry International
T Nakabayashi, H Ikezawa
© 2021 Meta ULC. All rights reserved