Abstract
As the first step toward understanding the identity and functions of thymic macrophages in situ, we examined the phenotypic heterogeneity of mouse thymic macrophages in tissue sections by the immunohistochemical double staining method with four monoclonal antibodies (F4/80, Mac-2, anti-CD32/16 and anti-I-A antibodies) as macrophage markers. Morphologically, three types of macrophages were identified: dendritic, round and flat-shaped. Dendritic macrophages were scattered throughout the thymus, and most of them were stained by all four markers. Among these macrophages, those at the cortico-medullary region (CMR) expressed a high intensity of CD32/16 antigen. Round macrophages were also distributed throughout the thymus; most of them, however, were localized in the cortico-medullary region to the medulla. These cells were F4/80-negative, Mac-2-positive, CD32/16-negative and I-A-positive. In contrast, round macrophages located at the cortex expressed F4/80. Flat-shaped macrophages were localized at the subcapsular region of the cortex where active lymphopoiesis was observed. This type was positive for F4/80 and CD32/16, but negative for Mac-2. Furthermore, most of the three types of thymic macrophages showed intense reactions of th...Continue Reading
Citations
Dec 1, 2006·Cell and Tissue Research·Yasunori SakakuraToshihiko Yajima
Feb 6, 2004·Blood Cells, Molecules & Diseases·Brian W SoperJane E Barker
Mar 4, 2000·Journal of Anatomy·S InoueA Tonosaki
Dec 16, 2000·Journal of Anatomy·M D Kendall, A G Clarke
Mar 21, 2013·Critical Reviews in Oncogenesis·Manoj Kumar Pandey, Gregory A Grabowski
Nov 25, 2003·Microscopy Research and Technique·Alberto VarasAngeles Vicente
Oct 18, 2008·Immunobiology·Taku WakimotoKiyoshi Takahashi
Oct 17, 2014·Immunological Reviews·Siamon GordonFernando Martinez Estrada
Sep 29, 2011·Immunity·Daigo HashimotoMiriam Merad
Apr 26, 2017·Human Vaccines & Immunotherapeutics·Fábio da Ressureição SgnottoJefferson Russo Victor
Mar 22, 2012·Toxicologic Pathology·Jerold E RehgJerrold M Ward
Apr 30, 2013·Molecular Medicine Reports·Lan-Tao LiuYu-Dong Xu
Sep 12, 2019·Viruses·Francesco AlbanoGiuseppe Fiume
Oct 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Haiguang WangKristin A Hogquist
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takuya IyodaYoshiro Kobayashi
Feb 2, 2021·European Journal of Immunology·S Thera LeeKristin A Hogquist