PMID: 7541465Apr 1, 1995Paper

Heterogeneity of neuronal nicotinic acetylcholine receptors in thin slices of rat medial habenula

The Journal of Physiology
J G ConnollyD Colquhoun

Abstract

1. Neuronal nicotinic acetylcholine receptors in slices of rat medial habenula were studied using patch clamp recording techniques. 2. Whole cell current responses to cytisine could be blocked by hexamethonium, as expected for nicotinic receptors. The whole cell current-voltage relations were linear at negative membrane potentials, but showed strong inward rectification when chloride currents were minimized. 3. When 1 mM Ca2+ (0 mM Mg2+) was present in the external recording solution, the single channel conductances elicited by acetylcholine or nicotine in twenty patches were in the range 39-58 pS, with a mean of 47 pS. There appeared to be at least two groups of conductances. 4. In the open point amplitude distributions of three patches, the most common amplitude corresponded to 41 pS (81% of the area). In another four patches the most common amplitude corresponded to a mean conductance of 51 pS (83% of the area). Direct transitions between open levels were rare. 5. Channel closed times were not significantly different for the two conductance groups. However, for the four patches with predominantly 51 pS openings, the means of the distributions of open times longer than two filter rise times averaged 5.8 ms. Those patches with...Continue Reading

Citations

Jan 10, 1998·British Journal of Pharmacology·P J Covernton, J G Connolly
Jan 5, 2002·Journal of Receptor and Signal Transduction Research·J G Connolly, C Kennedy
Oct 20, 2006·Journal of Neurophysiology·Xiaochuan Guo, Robin A J Lester
Sep 16, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alberto Pérez-AlvarezAlmudena Albillos
Jul 4, 1998·Brain Research. Brain Research Reviews·J P ChangeuxM Zoli
Sep 22, 1995·Science·L Sivilotti, D Colquhoun

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