PMID: 7029548Sep 1, 1981Paper

Heterogeneity of newly inserted and preexisting murein in the sacculus of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
Miguel A de Pedro, U Schwarz

Abstract

In vivo studies of murein biosynthesis show that newly synthesized murein unexpectedly differs in its chemical composition from preexisting murein. New murein is loosely crosslinked with the preexisting sacculus; in a maturation process involving further transpeptidation, the final stage of crosslinkage in murein is achieved. Newly inserted murein initially carries pentapeptide subunits, which are the donor of the secondary transpeptidation reaction. In mutants with defective penicillin-binding protein 4 the secondary transpeptidation step is abolished. Uncoupling of the secondary transpeptidation reaction from crosslink formation during the initial insertion of new murein was also found in a mutant with a defect in lipoprotein biosynthesis. We conclude that the initial transpeptidation of murein and crosslink formation during the maturation of newly inserted murein are catalyzed by two different enzyme systems.

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Citations

Jan 1, 1983·Molecular & General Genetics : MGG·K IidaU Schwarz
Jan 1, 1984·Pharmacology & Therapeutics·J B Ward
Jan 1, 1985·Pharmacology & Therapeutics·D J Tipper
Jan 1, 1990·Research in Microbiology·J V Höltje, B Glauner
Jan 1, 1990·Research in Microbiology·N NanningaC L Woldringh
Jan 1, 1985·Annales De L'Institut Pasteur. Microbiology·J T Park, L G Burman
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L G Burman, J T Park
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E J de la RosaD Vázquez
Jul 31, 1990·European Journal of Biochemistry·M A Snowden, H R Perkins
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Kevin D Young
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Jul 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lam T NguyenGrant J Jensen
Jan 1, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kok-Fai KongKalai Mathee
Jan 16, 2008·FEMS Microbiology Reviews·Waldemar VollmerMiguel A de Pedro
Jul 15, 2015·PLoS Genetics·Frédéric J VeyrierIvo G Boneca
Dec 1, 2012·Current Opinion in Microbiology·Kerwyn Casey HuangJoshua W Shaevitz
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J T Park
Jan 1, 1983·Journal of Receptor Research·J ZorzopulosL M Kozloff
Sep 1, 1988·Microbiological Reviews·A L Koch
Sep 1, 1992·Journal of Bacteriology·M CaparrósM A de Pedro
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J V Höltje
May 1, 1997·Journal of Bacteriology·M A de PedroH Schwarz
Oct 1, 1985·Journal of Bacteriology·R A NicholasY Hirota
Dec 1, 1982·Journal of Bacteriology·K SchallerV Braun
Nov 1, 1982·Journal of Bacteriology·J K Broome-Smith, B G Spratt
Oct 1, 1983·Journal of Bacteriology·E W GoodellU Schwarz
Oct 1, 1983·Journal of Bacteriology·E W Goodell, U Schwarz

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