Heterogeneity of Orientia tsutsugamushi genotypes in field-collected trombiculid mites from wild-caught small mammals in Thailand

PLoS Neglected Tropical Diseases
Ratree TakhampunyaSilas A Davidson

Abstract

Trombiculid mites are the vectors of scrub typhus, with infected larval mites (chiggers) transmitting the causative agent, Orientia tsutsugamushi, during feeding. Co-existence of multiple O. tsutsugamushi strains within infected mites has previously been reported in naturally infected, laboratory-reared mite lines using molecular methods to characterize the 56-kDa type-specific antigen (TSA) gene. In the current study, more advanced next-generation sequencing technology was used to reveal the heterogeneity of O. tsutsugamushi genotypes in field-collected trombiculid mites from rodents and small mammals in scrub typhus-endemic areas of Thailand. Twenty-eight trombiculid mites collected from 10 small mammals were positive for O. tsutsugamushi, corresponding to a prevalence rate of 0.7% within the mite population. Twenty-four of the infected mites were Leptotrombidium spp., indicating that this genus is the main vector for O. tsutsugamushi transmission in Thailand. In addition, O. tsutsugamushi was detected in the mite genera Ascoschoengastia, Blankaartia, Gahrliepia, and Lorillatum. Of the 10 infested small animal hosts, six had 2-10 infected mites feeding at the time of collection. Deep sequencing was used to characterize mixed ...Continue Reading

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Citations

May 6, 2019·Tropical Medicine and Infectious Disease·Daryl J KellyAllen L Richards
Oct 31, 2020·Tropical Medicine and Infectious Disease·Daniel H ParisAllen L Richards
Nov 18, 2020·The Korean Journal of Parasitology·Jung Wook ParkJae Il Lee
Mar 28, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ivo ElliottElizabeth M Batty

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Datasets Mentioned

BETA
MH290189
MH290190
MH290191
MH290192
MH290194
MH290195
MH290196
MH290199

Methods Mentioned

BETA
genotyping
PCR
Assay

Software Mentioned

R
CLC Genomics Workbench
gplots R package
Gplots
MUSCLE
BioEdit
package
DNADist DNA
MEGA

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