Heterogeneity of Phenotype and Function Reflects the Multistage Development of T Follicular Helper Cells

Frontiers in Immunology
Marta TrübDavid Gray

Abstract

T follicular helper cells (Tfh) provide crucial signals for germinal center (GC) formation, but Tfh populations are heterogeneous. While PD1(hi) Tfh are important in the GC response, the function of the PD1(lo) Tfh-like subset is unknown. We show that these cells, like the PD1(hi) GC-Tfh, depend upon B cells; however, their entry to follicles is independent of CXCR5 or cognate interactions with B cells. The differentiation into PD1(hi) Tfh is dependent on MHC class II interactions with B cells and requires CXCR5. Our data suggest a Tfh differentiation pathway that is initially B cell-independent, then dependent on non-cognate B cell interactions, and finally following cognate interaction with B cells and CXCR5-ligands allows the formation of GC-Tfh. The PD1(lo) Tfh-like cells make early cytokine responses and may represent precursors of CD4 memory cells.

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Citations

Nov 23, 2017·Frontiers in Immunology·Lin YanCarla C Baan
Sep 7, 2018·Clinical Science·Claire E GustafsonJörg J Goronzy
Aug 2, 2018·Frontiers in Immunology·Noémie GensousChristophe Richez
Nov 18, 2020·International Journal of Molecular Sciences·Hannah LawC Mee Ling Munier
May 15, 2021·Frontiers in Immunology·Amber Papillion, André Ballesteros-Tato
Sep 4, 2021·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Yvanna Louise Di Christine OliveiraSilvio Santana Dolabella

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Methods Mentioned

BETA
flow cytometry
FACS
PMA
FCS
PCR
chip

Software Mentioned

BD FACS
limma
R
OpenLab
Bioconductor
limma Bioconductor
rma
FlowJo
- Diva

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