PMID: 590201Oct 1, 1977

Heterogeneity of rat luteinizing hormone revealed by radioimmunoassay and electrofocusing studies

Endocrinologia Japonica
K Wakabayashi

Abstract

Radioimmunoassay of rat luteinizing hormone (LH) in the sera and the anterior pituitaries of normal and castrated males and proestrous females employing different batches of anti-LH sera gave the results suggesting heterogeneity of immunoreactive LH (IR-LH). Gel filtration analyses of anterior pituitary extract and pooled sera failed to give positive evidence for the heterogeneity. But when the extracts of normal rat anterior pituitaries were electrofocused and the fractions were assayed, the pituitary glycoprotein hormones, LH, FSH and TSH, were found to consist of several components with different isoelectric points. In electrofocusing patterns of IR-LH, distinct peaks were observed at pH 8.5 (component A), 8.8 (component B), 9.1 (component C) and 9.3-9.4 (component D), and less distinct peaks were observed at pH 7.9 (component A'), 9.6 (component E) and 9.8 (component F). There seemed to be some sexual difference in the relative amounts of these components. Orchiectomy caused marked changes in electrofocusing patterns of IR-LH, i.e., increase of components A', A, B, C and D. The blockade of LH release by estradiol benzoate and progesterone treatment in orchiectomized rats caused a marked increase of component A. At the proes...Continue Reading

Citations

Sep 1, 1984·Journal of Steroid Biochemistry·M HattoriM L Dufau
Dec 1, 1987·Molecular and Cellular Endocrinology·P J SnyderM Karowe
Jun 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·K A KrishnanJ M Bahr
Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·A IwasawaM Kamiyoshi
Apr 1, 1992·International Journal of Andrology·Y ItoH Mori
Apr 1, 1994·Journal of Endocrinological Investigation·S CostagliolaP Carayon
Mar 1, 1983·General and Comparative Endocrinology·A A KuznetzovE Burzawa-Gerard
Jul 1, 1985·General and Comparative Endocrinology·S TanakaK Wakabayashi
Mar 15, 1985·Biochemical and Biophysical Research Communications·M HattoriK Wakabayashi
Feb 1, 1987·Baillière's Clinical Endocrinology and Metabolism·M L Dufau, J D Veldhuis
Dec 1, 1983·Journal of Endocrinological Investigation·P MarramaG F Baraghini

Related Concepts

Castration
Molecular Sieve Chromatography
Isoelectric Focusing
Luteozyman
Pituitary Gland, Anterior
Radioimmunoassay

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