Heterogeneity of SOX9 and HNF1β in Pancreatic Ducts Is Dynamic

Stem Cell Reports
Habib RezanejadSusan Bonner-Weir

Abstract

Pancreatic duct epithelial cells have been suggested as a source of progenitors for pancreatic growth and regeneration. However, genetic lineage-tracing experiments with pancreatic duct-specific Cre expression have given conflicting results. Using immunofluorescence and flow cytometry, we show heterogeneous expression of both HNF1β and SOX9 in adult human and murine ductal epithelium. Their expression was dynamic and diminished significantly after induced replication. Purified pancreatic duct cells formed organoid structures in 3D culture, and heterogeneity of expression of Hnf1β and Sox9 was maintained even after passaging. Using antibodies against a second cell surface molecule CD51 (human) or CD24 (mouse), we could isolate living subpopulations of duct cells enriched for high or low expression of HNF1β and SOX9. Only the CD24high (Hnfβhigh/Sox9high) subpopulation was able to form organoids.

Citations

Sep 20, 2018·Current Stem Cell Research & Therapy·Aravinth P JawaharAppakalai N Balamurugan
Oct 22, 2019·Stem Cells Translational Medicine·Valentina VillaniLaura Perin
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina MameishviliAnthony Gavalas
Dec 15, 2018·Current Protocols in Cell Biology·Habib RezanejadSusan Bonner-Weir
Mar 15, 2019·In Vitro Cellular & Developmental Biology. Animal·Neil C TalbotWesley M Garrett
Jun 4, 2020·Pathology Oncology Research : POR·Michaela BártůPavel Dundr
Feb 16, 2021·World Journal of Stem Cells·Kang-Li WangRui Wei
Jan 27, 2021·Cellular and Molecular Gastroenterology and Hepatology·Deepthi Y TulasiAdam D Gracz

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Methods Mentioned

BETA
fluorescence-activated cell sorting
FACS
Flow cytometry
pancreatectomy
light microscopy

Software Mentioned

Statview
FlowJo

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