Abstract
Each skeletal muscle contains a fixed ratio of fast and slow myofibers that are distributed in a stereotyped pattern to achieve a specific motor function. How myofibers are specified during development and regeneration is poorly understood. Here we address this question using transgenic reporter mice that indelibly mark the myofiber lineages based on activation of fast or slow myosin. Lineage tracing indicates that during development all muscles have activated the fast myosin gene Myl1, but not the slow myosin gene Myh7, which is activated in all slow but a subset of fast myofibers. Similarly, most nascent myofibers do not activate Myh7 during fast muscle regeneration, but the ratio and pattern of fast and slow myofibers are restored at the completion of regeneration. At the single myofiber level, most mature fast myofibers are heterogeneous in nuclear composition, manifested by mosaic activation of Myh7. Strikingly, Myh7 is activated in a subpopulation of proliferating myoblasts that co-express the myogenic progenitor marker Pax7. When induced to differentiate, the Myh7-activated myoblasts differentiate more readily than the non-activated myoblasts, and have a higher tendency, but not restricted, to become slow myotubes. Toget...Continue Reading
References
Feb 1, 1991·Developmental Biology·J L Feldman, F E Stockdale
Feb 1, 1987·Developmental Biology·L C Cerny, E Bandman
Oct 23, 1997·The Journal of Clinical Investigation·M C ColbertJ Robbins
Sep 25, 2003·Biochimie·David J Timson
Apr 15, 2004·The Journal of Biological Chemistry·Stephanie A ParsonsJeffery D Molkentin
Jul 28, 2004·The Journal of Cell Biology·Peter S ZammitJonathan R Beauchamp
Oct 16, 2004·PLoS Biology·Juleen R Zierath, John A Hawley
Oct 27, 2004·Developmental Biology·Hugo C Olguin, Bradley B Olwin
Jun 8, 2006·Annual Review of Biochemistry·Rhonda Bassel-Duby, Eric N Olson
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Jan 26, 2008·Trends in Molecular Medicine·Shihuan Kuang, Michael A Rudnicki
Nov 20, 2009·Developmental Cell·Eva van RooijEric N Olson
Dec 22, 2009·Nature Neuroscience·Linda MadisenHongkui Zeng
Jun 15, 2010·The International Journal of Biochemistry & Cell Biology·Leonidas G Karagounis, John A Hawley
Aug 5, 2010·Cell Stem Cell·Kostandin V PajciniHelen M Blau
Dec 3, 2010·PloS One·Jolena N WaddellShihuan Kuang
Sep 8, 2011·Genesis : the Journal of Genetics and Development·Joe V ChakkalakalJoshua R Sanes
Oct 21, 2011·Physiological Reviews·Stefano Schiaffino, Carlo Reggiani
Jan 26, 2012·Development·Susan M Abmayr, Grace K Pavlath
Feb 4, 2012·Journal of Molecular and Cellular Cardiology·Jinghai Chen, Da-Zhi Wang
Jul 27, 2012·Development·Frederic Relaix, Peter S Zammit
Jul 31, 2013·Journal of Animal Science·H ZhuD E Gerrard
Citations
Dec 9, 2016·American Journal of Physiology. Cell Physiology·Fuminori KawanoNaoya Nakai
Jul 29, 2017·Bio-protocol·Chao WangShihuan Kuang
Nov 30, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vered RazErik Ben van den Akker
Apr 2, 2019·Frontiers in Physiology·Cody T HaunMichael D Roberts
Oct 11, 2020·Nature Communications·Matthieu Dos SantosPascal Maire
Apr 21, 2020·International Immunopharmacology·Liulei ZhangJinghua Wang
Nov 4, 2021·Molecular Systems Biology·Kazumitsu MaeharaYasuyuki Ohkawa
May 12, 2021·Spine·Gregory JamesPaul W Hodges
May 23, 2017··Chao WangFeng Yue