Heterogeneous activation of a slow myosin gene in proliferating myoblasts and differentiated single myofibers

Developmental Biology
Jing-Hua WangShihuan Kuang

Abstract

Each skeletal muscle contains a fixed ratio of fast and slow myofibers that are distributed in a stereotyped pattern to achieve a specific motor function. How myofibers are specified during development and regeneration is poorly understood. Here we address this question using transgenic reporter mice that indelibly mark the myofiber lineages based on activation of fast or slow myosin. Lineage tracing indicates that during development all muscles have activated the fast myosin gene Myl1, but not the slow myosin gene Myh7, which is activated in all slow but a subset of fast myofibers. Similarly, most nascent myofibers do not activate Myh7 during fast muscle regeneration, but the ratio and pattern of fast and slow myofibers are restored at the completion of regeneration. At the single myofiber level, most mature fast myofibers are heterogeneous in nuclear composition, manifested by mosaic activation of Myh7. Strikingly, Myh7 is activated in a subpopulation of proliferating myoblasts that co-express the myogenic progenitor marker Pax7. When induced to differentiate, the Myh7-activated myoblasts differentiate more readily than the non-activated myoblasts, and have a higher tendency, but not restricted, to become slow myotubes. Toget...Continue Reading

References

Apr 15, 2004·The Journal of Biological Chemistry·Stephanie A ParsonsJeffery D Molkentin
Jul 28, 2004·The Journal of Cell Biology·Peter S ZammitJonathan R Beauchamp
Oct 16, 2004·PLoS Biology·Juleen R Zierath, John A Hawley
Jun 8, 2006·Annual Review of Biochemistry·Rhonda Bassel-Duby, Eric N Olson
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Jan 26, 2008·Trends in Molecular Medicine·Shihuan Kuang, Michael A Rudnicki
Jun 15, 2010·The International Journal of Biochemistry & Cell Biology·Leonidas G Karagounis, John A Hawley
Dec 3, 2010·PloS One·Jolena N WaddellShihuan Kuang
Sep 8, 2011·Genesis : the Journal of Genetics and Development·Joe V ChakkalakalJoshua R Sanes
Oct 21, 2011·Physiological Reviews·Stefano Schiaffino, Carlo Reggiani
Jan 26, 2012·Development·Susan M Abmayr, Grace K Pavlath
Feb 4, 2012·Journal of Molecular and Cellular Cardiology·Jinghai Chen, Da-Zhi Wang
Jul 31, 2013·Journal of Animal Science·H ZhuD E Gerrard

❮ Previous
Next ❯

Citations

Dec 9, 2016·American Journal of Physiology. Cell Physiology·Fuminori KawanoNaoya Nakai
Nov 30, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vered RazErik Ben van den Akker
Oct 11, 2020·Nature Communications·Matthieu Dos SantosPascal Maire
Apr 21, 2020·International Immunopharmacology·Liulei ZhangJinghua Wang
Nov 4, 2021·Molecular Systems Biology·Kazumitsu MaeharaYasuyuki Ohkawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.