Abstract
The mineralization process is crucial to the load-bearing characteristics of the bone extracellular matrix. In this work, we have studied the spatiotemporal dynamics of mineral deposition by human bone marrow mesenchymal stem cells differentiating toward osteoblasts promoted by the presence of exogenous hydroxyapatite nanoparticles. At the molecular level, the added nanoparticles positively modulated the expression of bone-specific markers and enhanced calcified matrix deposition during osteogenic differentiation. The nucleation, growth and spatial arrangement of newly deposited hydroxyapatite nanocrystals have been evaluated using scanning micro X-ray diffraction and scanning micro X-ray fluorescence. As leading results, we have found the emergence of a complex scenario where the spatial organization and temporal evolution of the process exhibit heterogeneous and self-organizing dynamics. At the same time the possibility of controlling the differentiation kinetics, through the addition of synthetic nanoparticles, paves the way to empower the generation of more structured bone scaffolds in tissue engineering and to design new drugs in regenerative medicine.
References
Jun 1, 1989·The Anatomical Record·P V Hauschka, F H Wians
Mar 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D J RickardB L Riggs
May 30, 1997·Cell·P DucyG Karsenty
Jul 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A K MajorsG F Muschler
Apr 2, 1999·Science·M F PittengerD R Marshak
Dec 26, 2006·Journal of Cellular Physiology·M E BernardoF Locatelli
Oct 3, 2007·Cancer Research·Maria Ester BernardoFranco Locatelli
Feb 22, 2008·Nature·Vincent F M Segers, Richard T Lee
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tetsuya MatsuguchiTomokazu Ohnishi
Jun 27, 2009·Science·Thaddeus S Stappenbeck, Hiroyuki Miyoshi
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Jan 22, 2011·Nature Reviews. Molecular Cell Biology·César Nombela-ArrietaLeslie E Silberstein
Aug 9, 2011·ACS Nano·Seon NamgungSeunghun Hong
Jun 9, 2012·Acta Biomaterialia·G CampiA Cedola
Aug 6, 2013·BioResearch Open Access·Gabriele CeccarelliLivia Visai
Oct 22, 2013·Biology Open·Omar F ZouaniMarie-Christine Durrieu
Apr 29, 2014·Scientific Reports·Paul BogdanRadu Marculescu
May 17, 2014·Archives of Biochemistry and Biophysics·Mattia CapulliNadia Rucci
Jun 26, 2014·Scientific Reports·Nicola PocciaAntonio Bianconi
Jul 30, 2014·Scientific Reports·Yanjiao HanWenguang Liu
Jun 7, 2015·Acta Biomaterialia·G CampiA Cedola
Sep 19, 2015·Nature·G CampiA Ricci
Dec 19, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Matteo FrasnelliLivia Visai
Jan 1, 2016·Nanomaterials·Marco VercellinoLivia Visai
Citations
Jan 24, 2019·Nanoscale·Rui ZhaoXingdong Zhang
Feb 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tianming DuYubo Fan
Nov 22, 2019·Scientific Reports·F CristofaroL Visai
Jun 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Honglin LuoYizao Wan
Jul 3, 2020·Frontiers in Bioengineering and Biotechnology·Giulia MontagnaLouis Gerstenfeld
Dec 15, 2020·Journal of Applied Biomaterials & Functional Materials·Yuan-Ming GengYu-Yu Yuan
Mar 8, 2020·Biochimica Et Biophysica Acta. Biomembranes·Michael Di GioacchinoGaetano Campi
Jun 4, 2021·International Journal of Nanomedicine·Qi LiuHong Yu
Jul 3, 2021·Nanomaterials·Riccardo Di SantoGabriele Ciasca