Heterogeneous and self-organizing mineralization of bone matrix promoted by hydroxyapatite nanoparticles

Nanoscale
G CampiG Rea

Abstract

The mineralization process is crucial to the load-bearing characteristics of the bone extracellular matrix. In this work, we have studied the spatiotemporal dynamics of mineral deposition by human bone marrow mesenchymal stem cells differentiating toward osteoblasts promoted by the presence of exogenous hydroxyapatite nanoparticles. At the molecular level, the added nanoparticles positively modulated the expression of bone-specific markers and enhanced calcified matrix deposition during osteogenic differentiation. The nucleation, growth and spatial arrangement of newly deposited hydroxyapatite nanocrystals have been evaluated using scanning micro X-ray diffraction and scanning micro X-ray fluorescence. As leading results, we have found the emergence of a complex scenario where the spatial organization and temporal evolution of the process exhibit heterogeneous and self-organizing dynamics. At the same time the possibility of controlling the differentiation kinetics, through the addition of synthetic nanoparticles, paves the way to empower the generation of more structured bone scaffolds in tissue engineering and to design new drugs in regenerative medicine.

References

Jun 1, 1989·The Anatomical Record·P V Hauschka, F H Wians
Mar 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D J RickardB L Riggs
Jul 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A K MajorsG F Muschler
Apr 2, 1999·Science·M F PittengerD R Marshak
Feb 22, 2008·Nature·Vincent F M Segers, Richard T Lee
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tetsuya MatsuguchiTomokazu Ohnishi
Jun 27, 2009·Science·Thaddeus S Stappenbeck, Hiroyuki Miyoshi
Jun 27, 2009·Science·Dennis E DischerPeter W Zandstra
Jan 22, 2011·Nature Reviews. Molecular Cell Biology·César Nombela-ArrietaLeslie E Silberstein
May 17, 2014·Archives of Biochemistry and Biophysics·Mattia CapulliNadia Rucci
Jul 30, 2014·Scientific Reports·Yanjiao HanWenguang Liu
Dec 19, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Matteo FrasnelliLivia Visai

❮ Previous
Next ❯

Citations

Feb 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tianming DuYubo Fan
Jun 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Honglin LuoYizao Wan
Jul 3, 2020·Frontiers in Bioengineering and Biotechnology·Giulia MontagnaLouis Gerstenfeld
Dec 15, 2020·Journal of Applied Biomaterials & Functional Materials·Yuan-Ming GengYu-Yu Yuan
Mar 8, 2020·Biochimica Et Biophysica Acta. Biomembranes·Michael Di GioacchinoGaetano Campi

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
Fluorescence
density gradient centrifugation
FACS
dynamic light scattering
X ray
PCR
enzyme-linked immunosorbent assay
Protein Assay

Software Mentioned

SμXRD
nHap

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.