PMID: 9438139Jan 23, 1998Paper

Heterogeneous behavior of cells with respect to induction of retrograde transport from the trans-Golgi network to the Golgi upon inhibition of the vacuolar proton pump

European Journal of Cell Biology
A W van WeertWillem Stoorvogel

Abstract

We investigated whether the vacuolar proton pump is required for proper vesicular trafficking through the trans-Golgi network. Previously, we provided evidence that endocytic transport to early and late endosomes, as well as recycling from endosomes to the plasma membrane, but not transport to lysosomes, continued in the presence of bafilomycin A1, a specific inhibitor of the vacuolar proton pump (Van Weert et al., J. Cell Biol. 130, 821-834 (1995)). Following endocytosis, recycling of cell surface glycoproteins occurs directly from endosomes to the plasma membrane, but also involves trafficking via the trans-Golgi network. We now used transferrin conjugated to horseradish peroxidase (Tf/HRP) to label and monitor the itinerary of the transferrin receptor using electron microscopy. In the presence of bafilomycin A1, transport of Tf/HRP to the trans-Golgi network continued. However, in a limited percentage of cells bafilomycin A1 induced transport of Tf/HRP into the Golgi stack. Retrograde transport into the Golgi stack was analyzed further in cells that were additionally treated with Brefeldin A, a drug that induces a redistribution of Golgi, but not trans-Golgi network markers, to the endoplasmic reticulum and the nuclear envel...Continue Reading

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