Heterogeneous calcium flux in peripheral T cell subsets revealed by five-color flow cytometry using log-ratio circuitry

Cytometry
M RoedererL A Herzenberg

Abstract

Calcium flux measurements of different subpopulations of cells by flow cytometry are important in understanding complex interactions in the immune system. This paper discusses the use of the difference of Log signals as a preferred method for obtaining this information simultaneously with other immunofluorescence parameters. We describe simple modifications to a commercial instrument that enables the measurement of calcium flux in addition to three immunofluorescence parameters. Finally, we show an application of this technique to measuring calcium flux of T cell subsets in human blood. We show that different subsets of peripheral CD4 T cells have significantly different capabilities to flux calcium after CD3 stimulation. These differences are related to the functional capacities of the cells within these subsets.

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M R LokenL A Herzenberg
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May 1, 1995·The Journal of Clinical Investigation·M RoedererL A Herzenberg

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Citations

Jul 11, 2013·Applied Microbiology and Biotechnology·Michael MeyerJohanna-Gabriela Walter
Feb 23, 1999·Human Immunology·P J MacardleH Zola
Apr 1, 1997·The Journal of Clinical Investigation·M RoedererL A Herzenberg
Jun 28, 2008·Blood·Siegfried Kohler, Andreas Thiel
May 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kaimei SongJoshua M Farber
Feb 24, 2016·The Journal of Clinical Investigation·Theo van den BroekFemke van Wijk
Jan 13, 2009·Immunology·Pratip K ChattopadhyayMario Roederer
Jun 3, 2015·Nature Reviews. Rheumatology·Joerg ErmannSoumya Raychaudhuri
Aug 3, 2004·Nature Reviews. Immunology·Stephen P PerfettoMario Roederer
Jun 21, 2006·Nature Immunology·Leonore A HerzenbergDavid R Parks
Apr 29, 2006·Violence and Victims·Sarah K Spilman
Sep 12, 2006·Visual Neuroscience·Mikel A AldabaDavid H Foster
Jan 29, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobutoshi OtaYo Tanaka
Feb 15, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy C PalinDavid B Lewis
Feb 7, 2001·Journal of the National Cancer Institute. Monographs·D T ScaddenT Cheng
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Oct 29, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongwei H ZhangJoshua M Farber

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