PMID: 11897837Mar 19, 2002Paper

Heterogeneous expression of KChIP2 isoforms in the ferret heart

The Journal of Physiology
Sangita P PatelHarold C Strauss

Abstract

Kv4 channels are believed to underlie the rapidly recovering cardiac transient outward current (I(to)) phenotype. However, heterologously expressed Kv4 channels fail to fully reconstitute the native current. Kv channel interacting proteins (KChIPs) have been shown to modulate Kv4 channel function. To determine the potential involvement of KChIPs in the rapidly recovering I(to), we cloned three KChIP2 isoforms (designated fKChIP2, 2a and 2b) from the ferret heart. Based upon immunoblot data suggesting the presence of a potential endogenous KChIP-like protein in HEK 293, CHO and COS cells but absence in Xenopus oocytes, we coexpressed Kv4.3 and the fKChIP2 isoforms in Xenopus oocytes. Functional analysis showed that while all fKChIP2 isoforms produced a fourfold acceleration of recovery kinetics compared to Kv4.3 expressed alone, only fKChIP2a produced large depolarizing shifts in the V(1/2) of steady-state activation and inactivation as seen for the native rapidly recovering I(to). Analysis of RNA and protein expression of the three fKChIP2 isoforms in ferret ventricles showed that fKChIP2b was most abundant and was expressed in a gradient paralleling the rapidly recovering I(to) distribution. Ferret KChIP2 and 2a were expressed...Continue Reading

References

May 18, 1999·Cardiovascular Research·L FranquezaD J Snyders
May 4, 2000·American Journal of Physiology. Cell Physiology·Y A KuryshevB A Wible
Nov 23, 2000·The Journal of Biological Chemistry·E K YangK Takimoto
Mar 27, 2001·Biochemical and Biophysical Research Communications·S OhyaY Imaizumi
May 10, 2001·Journal of Molecular and Cellular Cardiology·G Y OuditP H Backx

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Citations

Jul 9, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·F FischerE P Scholz
Jan 24, 2007·Pflügers Archiv : European journal of physiology·Jobst-Hendrik SchultzHeimo Ehmke
May 25, 2005·Molecular and Cellular Neurosciences·Xiaomeng RenKoichi Takimoto
Jun 11, 2005·Journal of Molecular and Cellular Cardiology·Jobst-Hendrik SchultzHeimo Ehmke
May 10, 2005·Journal of Molecular and Cellular Cardiology·Huilin LiJeanne M Nerbonne
May 10, 2005·Journal of Molecular and Cellular Cardiology·Sophie Demolombe
Oct 24, 2003·Molecular and Cellular Neurosciences·Diane Van HoorickDirk J Snyders
Feb 19, 2009·Learning & Memory·Jon C AlexanderLaura A Schrader
Apr 10, 2009·Molecular Pharmacology·Christopher V DeSimoneMichael J Morales
Aug 14, 2009·American Journal of Physiology. Cell Physiology·Chang XieHarold C Strauss
Oct 8, 2009·Circulation. Arrhythmia and Electrophysiology·Stefan WagnerLars S Maier
Nov 21, 2008·PloS One·Matthew R Skerritt, Donald L Campbell
Oct 24, 2006·Nature Structural & Molecular Biology·Marta PiolettiDaniel L Minor
Nov 16, 2002·The Journal of Physiology·Sangita P PatelHarold C Strauss
May 26, 2010·The Journal of Physiology·Daniel I LevySteve A N Goldstein
Apr 28, 2012·Journal of Molecular and Cellular Cardiology·Eman S H Abd AllahMark R Boyett
Aug 2, 2011·Journal of Molecular and Cellular Cardiology·E P ScholzE Zitron
May 21, 2009·The European Journal of Neuroscience·Plamena R Angelova, Wolfgang S Müller
Jul 31, 2007·Protein Science : a Publication of the Protein Society·Sudarshan Rajagopal, Stephen B H Kent
Aug 2, 2002·Biochemical and Biophysical Research Communications·Shimin WangMichael J Morales
Jan 18, 2006·Biochemical and Biophysical Research Communications·Glenna C L BettRandall L Rasmusson
Oct 25, 2008·Biochimica Et Biophysica Acta·Matthew R Skerritt, Donald L Campbell
Oct 21, 2016·Progress in Biophysics and Molecular Biology·David McKinnon, Barbara Rosati
Jan 16, 2004·The Journal of Physiology·Sangita P PatelDonald L Campbell
Apr 27, 2004·The Journal of Physiology·Niels DecherMichael C Sanguinetti
Sep 26, 2002·FEBS Letters·Isabelle Deschênes, Gordon F Tomaselli
Jul 23, 2004·Physiological Reviews·Shari G BirnbaumLaura A Schrader
Jul 24, 2002·Circulation·Isabelle DeschênesGordon F Tomaselli
Apr 17, 2004·Circulation Research·Barbara Rosati, David McKinnon
Sep 27, 2005·Physiological Reviews·Jeanne M Nerbonne, Robert S Kass
Jun 2, 2006·American Journal of Physiology. Cell Physiology·Hong-Ling LiMichael J Morales
Apr 13, 2007·Physiological Reviews·Henk E D J Ter Keurs, Penelope A Boyden
Dec 22, 2007·The Journal of Physiology·Glenna C L Bett, Randall L Rasmusson
Aug 21, 2003·The Journal of Biological Chemistry·Xiaomeng RenKoichi Takimoto
Mar 9, 2004·The Journal of Physiology·Shimin WangHarold C Strauss

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