Heterogeneous gene expression and functional activity of ryanodine receptors in resistance and conduit pulmonary as well as mesenteric artery smooth muscle cells.

Journal of Vascular Research
Yun-Min ZhengYong-Xiao Wang

Abstract

Hypoxia causes heterogeneous contractile responses in resistance and conduit pulmonary as well as systemic (mesenteric) artery smooth muscle cells (RPASMCs, CPASMCs and MASMCs), but the underlying mechanisms are largely unknown. In this study, we aimed to investigate whether the gene expression and functional activity of ryanodine receptors (RyRs) would be different in these 3 cell types. RyR mRNA expression, Ca(2+) sparks and [Ca(2+)](i) were measured by real-time quantitative RT-PCR, laser scanning confocal microscopy and wide-field fluorescence microscopy, respectively. All 3 RyR subtype (RyR1, RyR2 and RyR3) mRNAs are expressed in RPASMCs, CPASMCs and MASMCs, but their expression levels are different. Spontaneous Ca(2+) sparks (functional events of RyRs) show distinct frequency, amplitude, duration, size and kinetics in these 3 cell types. Similarly, activation of RyRs by caffeine, 4-chloro-m-cresol or high K(+) induces differential Ca(2+) release. Moreover, hypoxia-induced increase in [Ca(2+)](i) is largest in MASMCs relative to CPSAMCs and smallest in RPASMCs. This study provides comprehensive evidence that RyRs are heterogeneous in gene expression and functional activity in RPASMCs, CPASMCs and MASMCs, which may contribu...Continue Reading

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Citations

Jul 30, 2008·Pflügers Archiv : European journal of physiology·Xiao-Qiang LiYong-Xiao Wang
Sep 22, 2009·Antioxidants & Redox Signaling·Yong-Xiao Wang, Yun-Min Zheng
Mar 2, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Erika B Westcott, William F Jackson
Sep 11, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Scott R HadleySean M Wilson
Jun 12, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kyle LeggettLarissa A Shimoda
Dec 4, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Vishal R YadavYong-Xiao Wang
May 15, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jian WangJ T Sylvester
Feb 22, 2013·American Journal of Physiology. Cell Physiology·Yun-Min ZhengYong-Xiao Wang
May 12, 2015·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M SnoeckE-J Kamsteeg
Aug 1, 2014·American Journal of Respiratory Cell and Molecular Biology·Suengwon LeeJames S K Sham
Nov 24, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christine P ShenSean M Wilson
Feb 2, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Vishal R YadavYong-Xiao Wang
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Sep 11, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Diana DahanEric Estève

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