PMID: 3769455Jan 1, 1986Paper

Heterogeneous humoral and hemocyte-associated lectins with N-acylaminosugar specificities from the blue crab, Callinectes sapidus Rathbun

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
F J CasselsG R Vasta

Abstract

Callinectes sapidus serum and hemocyte microsomal fraction agglutinated a panel of untreated and enzyme treated vertebrate erythrocytes and cultured lymphoid cell lines. Crossed absorption experiments suggested the presence of multiple specific lectins in the serum. The microsomal fraction showed a 35-fold increase in specific activity when compared to the hemocyte lysate suggesting that hemocyte lectins are membrane-associated. Agglutination by serum and hemocyte lectins was inhibited by low concentrations of N-acylamino compounds including sialic, N-acetylmuramic and N-acetylglutamic acids, GalNAc, GlcNAc, ManNAc, and glycoproteins and polysaccharides which contain these carbohydrates: bovine submaxillary mucin, human orosomucoid, porcine stomach mucin and colominic acid. Hemagglutination by lectins of both serum and hemocyte microsomal fraction required divalent cations as suggested by the reduction in hemagglutination titer in the presence of the chelators EDTA, EGTA, CDTA and citrate.

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Citations

Apr 1, 1996·Journal of Cellular Biochemistry·M ImschenetzkyM Puchi
Oct 1, 1995·Journal of Cellular Biochemistry·M ImschenetzkyM Montecino
Jun 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S TunkijjanukijJ A Olafsen
Jan 30, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Valentina MolchanovaPavel Lukyanov
Jun 22, 2010·Journal of Molecular Biology·Mario A BianchetL Mario Amzel
May 29, 2009·Innate Immunity·Lorena VazquezEdgar Zenteno
Jun 8, 2002·Archives of Biochemistry and Biophysics·Rajagopalan MaheswariMunusamy Arumugam

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