Heterogeneous nuclear ribonucleoprotein C1 may control miR-30d levels in endometrial exosomes affecting early embryo implantation.

Molecular Human Reproduction
N BalaguerF Vilella

Abstract

Is there a specific mechanism to load the microRNA (miRNA), hsa-miR-30d, into exosomes to facilitate maternal communication with preimplantation embryos? The heterogeneous nuclear ribonucleoprotein C1 (hnRNPC1) is involved in the internalization of endometrial miR-30d into exosomes to prepare for its subsequent incorporation into trophectoderm cells. Our group previously described a novel cell-to-cell communication mechanism involving the delivery of endometrial miRNAs from the maternal endometrium to the trophectoderm cells of preimplantation embryos. Specifically, human endometrial miR-30d is taken up by murine blastocysts causing the overexpression of certain genes involved in embryonic adhesion (Itb3, Itga7 and Cdh5) increasing embryo adhesion rates. Transfer of maternal miR-30d to preimplantation embryos was confirmed by co-culture of wild-type (WT) and miR-30d knockout (KO) murine embryos with primary cultures of human endometrial epithelial cells (hEECs) in which mir-30d was labeled with specific Molecular Beacon (MB) or SmartFlare probes. Potential molecules responsible for the miR-30d loading into exosomes were purified by pull-down analysis with a biotinylated form of miR-30d on protein lysates from human endometrial ...Continue Reading

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Citations

Oct 31, 2019·The Journal of Clinical Endocrinology and Metabolism·Alessia GrassoF Vilella
Apr 5, 2020·Genes·Anna Idelevich, Felipe Vilella
Jan 5, 2021·Journal of Extracellular Vesicles·Fabrizio FabbianoVito G D'Agostino
Jun 22, 2021·Frontiers in Endocrinology·Nan-Xing Jiang, Xue-Lian Li
Nov 11, 2021·Journal of Leukocyte Biology·Mrigya Babuta, Gyongyi Szabo

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