Heterogeneous nuclear ribonucleoprotein D/AUF1 interacts with heterogeneous nuclear ribonucleoprotein L.

Journal of Biosciences
Hong-Gyu ParkMieyoung Choi

Abstract

Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is one of the principal pre-mRNA-binding proteins found in human cells. The hnRNP L protein is fairly abundant. However,it is not restricted to the nucleus, and instead shuttles between the nucleus and the cytoplasm. It is composed of 558 amino acid residues and harbours four loosely conserved RNP-consensus RNA-binding domains. In an attempt to characterize the interaction occurring between cellular proteins and hnRNP L, yeast two-hybrid screening was conducted using a HeLa cDNA library. Some of the cDNA clones were found to harbour a partial human hnRNP D/AUF1 cDNA (GeneBank accession number NM_031369). In this study,we determined that hnRNP L interacts specifically with the hnRNP D/AUF1 in the yeast two-hybrid system. This interaction was verified via an in vitro pull-down assay.

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Citations

May 15, 2009·Biochimica Et Biophysica Acta·Michael Niepmann
Mar 21, 2009·Biochemical and Biophysical Research Communications·Dong-Hyoung LeeJeong-Hwa Lee
Jan 13, 2015·Virus Research·Selena M SaganPeter Sarnow
Mar 13, 2012·Journal of Proteomics·Marie-Aline PierrardFrédéric Silvestre
Mar 12, 2010·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Mi-Hyun LimJeong-Hwa Lee
Feb 21, 2018·BMC Research Notes·V S TompkinsW N Moss

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