Heterogeneous nuclear transfer embryos reconstructed by bovine oocytes and camel (Camelus bactrianus) skin fibroblasts and their subsequent development.

In Vitro Cellular & Developmental Biology. Animal
Huanmin Zhou, Zhenhua Guo

Abstract

This study reconstructed heterogeneous embryos using camel skin fibroblast cells as donor karyoplasts and the bovine oocytes as recipient cytoplasts to investigate the reprogramming of camel somatic cell nuclei in bovine oocyte cytoplasm and the developmental potential of the reconstructed embryos. Serum-starved skin fibroblast cells, obtained from adult camel, were electrically fused into enucleated bovine metaphase II (MII) oocytes that were matured in vitro. The fused eggs were activated by Inomycin with 2 mM/ml 6-dimethylaminopurine. The activated reconstructed embryos were cocultured with bovine cumulus cells in synthetic oviduct fluid supplemented with amino acid (SOFaa) and 10% fetal calf serum for 168 h. Results showed that 53% of the injected oocytes were successfully fused, 34% of the fused eggs underwent the first egg cleavage, and 100% of them developed to four- or 16-cell embryo stages. The first completed cleavage of xenonuclear transfer camel embryos occurred between 22 and 48 h following activation. This study demonstrated that the reconstructed embryos underwent the first embryonic division and that the reprogramming of camel fibroblast nuclei can be initiated in enucleated bovine MII oocytes.

References

Mar 7, 1996·Nature·K H CampbellI Wilmut
Feb 27, 1997·Nature·I WilmutK H Campbell
Dec 16, 1998·Science·Y KatoY Tsunoda
May 20, 1999·Nature Biotechnology·A BaguisiY Echelard
Jun 16, 1999·Molecular Reproduction and Development·G E Corley-Smith, B P Brandhorst
Aug 12, 1999·Seminars in Cell & Developmental Biology·K H Campbell
Aug 19, 2000·Science·A OnishiA C Perry
Feb 23, 2002·Nature·T ShinM Westhusin
May 31, 2003·Science·Gordon L WoodsBarry J Pate
Aug 9, 2003·Nature·Cesare GalliGiovanna Lazzari
Oct 17, 2003·Nature·G W Baxter, J S Olafsen

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