Heterogeneous rotational diffusion of a fluorescent probe in lipid monolayers

Structural Dynamics
Neda DadashvandChristina M Othon

Abstract

The rotational correlation time of the lipid probe 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) is measured using fluorescence anisotropy for two lipid species. We measure the rotational diffusion in a monolayer of 1,2-Didecanoyl-sn-glycero-3-phosphocholine (DPPC) which displays a phase transition at room temperature from the liquid-expanded to the liquid-condensed phase. The constant rotational diffusion of the probe throughout the phase transition reflects the measurement of dynamics in only the liquid-expanded phase. We contrast the dynamic changes during this phase coexistence to the continuous density increase observed in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at room temperature. We observe a non-exponential decay of the probe diffusion consistent with heterogeneity of the orientational dynamics.

References

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Citations

Nov 16, 2016·Physical Biology·Neda Dadashvand, Christina M Othon
May 26, 2016·The Journal of Physical Chemistry. B·Neha ShafiqueFrancis W Starr

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Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy

Software Mentioned

MEM
Labview

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