Heterogeneous transgene expression in the retinas of the TH-RFP, TH-Cre, TH-BAC-Cre and DAT-Cre mouse lines

Neuroscience
H E VuongN C Brecha

Abstract

Transgenic mouse lines are essential tools for understanding the connectivity, physiology and function of neuronal circuits, including those in the retina. This report compares transgene expression in the retina of a tyrosine hydroxylase (TH)-red fluorescent protein (RFP) mouse line with three catecholamine-related Cre recombinase mouse lines [TH-bacterial artificial chromosome (BAC)-, TH-, and dopamine transporter (DAT)-Cre] that were crossed with a ROSA26-tdTomato reporter line. Retinas were evaluated and immunostained with commonly used antibodies including those directed to TH, GABA and glycine to characterize the RFP or tdTomato fluorescent-labeled amacrine cells, and an antibody directed to RNA-binding protein with multiple splicing to identify ganglion cells. In TH-RFP retinas, types 1 and 2 dopamine (DA) amacrine cells were identified by their characteristic cellular morphology and type 1 DA cells by their expression of TH immunoreactivity. In the TH-BAC-, TH-, and DAT-tdTomato retinas, less than 1%, ∼ 6%, and 0%, respectively, of the fluorescent cells were the expected type 1 DA amacrine cells. Instead, in the TH-BAC-tdTomato retinas, fluorescently labeled AII amacrine cells were predominant, with some medium diameter ...Continue Reading

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Citations

Nov 14, 2015·Brain Structure & Function·Robert GábrielMárta Wilhelm
May 5, 2017·The Journal of Comparative Neurology·Luis Pérez de Sevilla MüllerNicholas C Brecha
May 23, 2017·Frontiers in Neuroanatomy·Luis Pérez de Sevilla MüllerNicholas C Brecha
Feb 13, 2020·Investigative Ophthalmology & Visual Science·Ling-Jie CuiShi-Jun Weng
Jun 8, 2021·Frontiers in Neuroanatomy·Ashley M ChenLuis Pérez de Sevilla Müller

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