Heterologous complementation studies reveal the solute transport profiles of a two-member nucleobase cation symporter 1 (NCS1) family in Physcomitrella patens

Plant Physiology and Biochemistry : PPB
Janet A MintonGeorge S Mourad

Abstract

As part of an evolution-function analysis, two nucleobase cation symporter 1 (NCS1) from the moss Physcomitrella patens (PpNCS1A and PpNCS1B) are examined--the first such analysis of nucleobase transporters from early land plants. The solute specificity profiles for the moss NCS1 were determined through heterologous expression, growth and radiolabeled uptake experiments in NCS1-deficient Saccharomyces cerevisiae. Both PpNCS1A and 1B, share the same profiles as high affinity transporters of adenine and transport uracil, guanine, 8-azaguanine, 8-azaadenine, cytosine, 5-fluorocytosine, hypoxanthine, and xanthine. Despite sharing the same solute specificity profile, PpNCS1A and PpNCS1B move nucleobase compounds with different efficiencies. The broad nucleobase transport profile of PpNCS1A and 1B differs from the recently-characterized Viridiplantae NCS1 in breadth, revealing a flexibility in solute interactions with NCS1 across plant evolution.

References

Dec 9, 2003·Journal of Plant Physiology·Claudio StasollaHiroshi Ashihara
Aug 17, 2004·The Journal of Biological Chemistry·Anja SchmidtMarcelo Desimone
May 4, 2006·Annual Review of Plant Biology·Rita ZrennerRalf Boldt
Sep 6, 2007·FEMS Microbiology Reviews·Areti Pantazopoulou, George Diallinas
Jan 6, 2009·FEBS Letters·Tyler A MansfieldGeorge S Mourad
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Citations

Feb 1, 2018·FEMS Microbiology Letters·Candace R AlexanderGeorge S Mourad
Aug 30, 2018·Microbial Pathogenesis·Amanda J Stoffer-BittnerNeil P Schultes

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