PMID: 11334785May 4, 2001Paper

Heterologous expression and properties of the gamma-subunit of the Fe-only hydrogenase from Thermotoga maritima

Biochimica Et Biophysica Acta
M F VerhagenM W Adams

Abstract

Thermotoga maritima is a hyperthermophilic bacterium that contains a complex, heterotrimeric (alpha(beta)gamma) Fe-only hydrogenase. Sequence analysis indicates that the gene encoding the smallest subunit (gamma), hydC, contains a predicted iron-sulfur cluster binding motif. However, characterization of the native gamma-subunit has been hampered by interference from and the inability to separate intact gamma-subunit from the other two subunits (alpha and beta). To investigate the function and properties of the isolated gamma-subunit, the gene encoding HydG was expressed in Escherichia coli. Two forms of the recombinant protein were obtained with molecular masses of 10 and 18 kDa, respectively. Both contained a single [2Fe-2S] cluster based on metal analysis, EPR and UV-visible spectroscopy. NH2-terminal sequencing revealed that the 10 kDa protein is a truncated form of the intact gamma-subunit and lacks the first 65 amino acid residues. The midpoint potential of the 18 kDa form was -356 mV at pH 7.0 and 25 degrees C, as measured by direct electrochemistry, and was pH dependent with a pK(ox) of 7.5 and a pK(red) of 7.7. The oxidized, recombinant gamma-subunit was stable at 80 degrees C under anaerobic conditions with a half-life...Continue Reading

References

Feb 1, 1979·Analytical Biochemistry·J S Chen, D K Blanchard
Mar 1, 1977·Bacteriological Reviews·R K ThauerK Decker
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Nov 5, 1990·Biochimica Et Biophysica Acta·M W Adams
Jul 1, 1989·European Journal of Biochemistry·W R Hagen
Oct 1, 1980·Canadian Journal of Microbiology·B R GlickS M Martin
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·V D SledT Ohnishi
Feb 1, 1996·Molecular and Biochemical Parasitology·E T Bui, P J Johnson
Jun 19, 1998·Biochimica Et Biophysica Acta·T Ohnishi
Dec 22, 1998·Nature·A AkhmanovaJ H Hackstein
Sep 22, 1999·European Journal of Biochemistry·T AlmeidaA Videira
Jan 1, 1953·Scandinavian Journal of Clinical and Laboratory Investigation·J GOA
Jan 1, 1995·World Journal of Microbiology & Biotechnology·P Schönheit, T Schäfer

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Citations

Dec 3, 2014·Biotechnology Advances·Simon K-M R RittmannSung Gyun Kang
Jul 24, 2008·Metabolic Engineering·Chung-Jung ChouRobert M Kelly
Dec 3, 2014·Biochimica Et Biophysica Acta·John W PetersMichael W W Adams
Apr 2, 2008·Annals of the New York Academy of Sciences·Francis E Jenney, Michael W W Adams
Apr 11, 2017·Nature Chemical Biology·Carolyn E LubnerJohn W Peters
Dec 6, 2001·FEBS Letters·J Meyer
Feb 28, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Guangliang PanMichael W W Adams
Dec 12, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Nipa ChongdarHideaki Ogata

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