Heterologous expression of a chimeric gene, OsDST-SRDX, enhanced salt tolerance of transgenic switchgrass (Panicum virgatum L.)

Plant Cell Reports
Huifang CenWanjun Zhang

Abstract

Overexpression of OsDST-SRDX chimeric gene in switchgrass promotes plant growth and improves the salt tolerance of transgenic switchgrass by improving its antioxidative ability. Switchgrass (Panicum virgatum L.) is a forage and model feedstock plant. To avoid competing with crops in arable land utilization, improving salt tolerance of switchgrass is required to use marginal saline land for switchgrass production. To improve salt tolerance of switchgrass, a chimeric DROUGHT AND SALT TOLERANCE (DST) gene OsDST-SRDX was constructed using the Chimeric REpressor gene-Silencing Technology (CRES-T), and introduced into switchgrass genome by Agrobacterium-mediated transformation. Compared to wild-type (WT) plants, OsDST-SRDX transgenic (TG) switchgrass plants showed wider leaves and thicker stems. They performed better under salt stress, had higher relative leaf water content, lower electrolyte leakage and lower malondialdehyde (MDA) content, and accumulated less Na+ and more K+ than WT controls. The transgenic plants had also higher activities of antioxidant enzymes associated with suppressed expressing of genes in H2O2 homeostasis, including glutathione S-transferase (GST2, GST6), cytochrome P450, peroxidase 24 precursor, and induced...Continue Reading

References

Feb 15, 2001·Trends in Plant Science·J K Zhu
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Keiichiro HiratsuMasaru Ohme-Takagi
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kazuya Yoshida
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Jo BrightSteven J Neill
Dec 31, 2005·Genetic Engineering·Viswanathan ChinnusamyJian-Kang Zhu
Jan 5, 2008·Science·Jorn P W Scharlemann, William F Laurance
May 1, 2008·Annual Review of Plant Biology·Rana Munns, Mark Tester
Dec 1, 2010·Plant Biotechnology Journal·Tomomi MitoKyoko Matsui
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chunxiang FuZeng-Yu Wang
Jul 2, 2011·Methods in Molecular Biology·Nobutaka MitsudaMasaru Ohme-Takagi
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·George S ChuckSarah Hake
Jun 27, 2012·Plant Cell Reports·Rengasamy Ramamoorthy, Prakash P Kumar
Apr 16, 2015·Journal of Experimental Botany·Rowan F SageBill Deen
Apr 4, 2018·Applied Biochemistry and Biotechnology·Fawad KaleemAbdul Rehman Khan
Oct 24, 2018·Plant Science : an International Journal of Experimental Plant Biology·Jianping YanWanjun Zhang
Jan 1, 2001·Plant Cell Reports·H A RichardsB V Conger
Mar 3, 2019·Plant Science : an International Journal of Experimental Plant Biology·Jinfang WangYang-Dong Guo
May 17, 2019·Plant Biotechnology Journal·Yanrong LiuWanjun Zhang
Sep 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shota YamauchiAtsushi Takemiya

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
X-ray
salt stress
Assay
scanning electron microscopy
MDA

Software Mentioned

SPSS
CRES

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.