Heterologous expression of adenovirus E3-gp19K in an E1a-deleted adenovirus vector inhibits MHC I expression in vitro, but does not prolong transgene expression in vivo

Gene Therapy
D B SchowalterM A Kay

Abstract

An E1a-deleted adenovirus vector constitutively expressing native adenovirus E3-gp19K (Ad.RSV-gp19K) was constructed in order to determine whether or not E3-gp19K mediated interference with antigen presentation would result in prolonged transgene expression in vivo. Cultured fibroblasts infected with Ad.RSV-gp19K produced a native size gp19K protein and had decreased cell surface levels of MHC I as shown by immunoprecipitation and flow cytometry. The congenic mouse strains Balb/b (H-2b MHC I with high gp19K affinity), Balb/k (H-2k MHC I with no gp19K affinity), and Balb/c (H-2d MHC I with moderate gp19K affinity) were chosen for in vivo experiments because of their range of gp19K affinities. Following transduction of mice form each strain with Ad.RSV-gp19K and AD/RSV-hAAT (a reporter adenovirus), or Ad/RSV-cFIX (control adenovirus) and Ad/RSV-hAAT, the level and duration of serum hAAT protein were unrelated to gp19K protein expression. Evaluation of MHC I abundance on hepatocytes following in vivo transduction demonstrated that recombinant adenovirus rapidly increased the abundance of surface MHC I molecules on hepatocytes, and surface MHC I molecules were reduced earlier and to a greater extent following wild-type adenovirus i...Continue Reading

Citations

Apr 20, 2004·Critical Reviews in Oncology/hematology·Frederik H E SchagenRob C Hoeben
May 20, 1998·Nature Genetics·C H MiaoM A Kay
Jan 26, 2000·Human Gene Therapy·J T BruderI Kovesdi
Jul 7, 2001·Human Gene Therapy·J MiA Lieber
Dec 22, 2004·Human Gene Therapy·Michael J McConnell, Michael J Imperiale
Oct 13, 2005·Genetic Vaccines and Therapy·Asami InoIchizo Kobayashi
Jan 18, 2005·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Stefania PiersantiIsabella Saggio
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jerome SchaackJohn Moorhead
Oct 6, 1999·Current Opinion in Biotechnology·K BenihoudM Perricaudet
Dec 24, 2003·International Reviews of Immunology·Drew L LichtensteinWilliam S M Wold
May 18, 2005·Molecular Genetics and Metabolism·David B SchowalterJerry Vockley
Mar 11, 2005·Advanced Drug Delivery Reviews·Zhi-Li XuTakao Hayakawa
Nov 18, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·A ScariaS C Wadsworth
Nov 18, 2000·Gene Therapy·X Danthinne, M J Imperiale
Feb 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P K SeitzC W Cooper
Nov 24, 2019·FEBS Letters·Jian GaoAnja Ehrhardt
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Seyma CharniMartin Villalba

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