Heterologous expression of alpha 1-integrin cDNA generates variable ligand specificities and alterations in cell shape

Cell Adhesion and Communication
L D WongM J Ignatius

Abstract

Integrins can mediate a diverse variety of functions that are regulated by unknown mechanisms. Integrin alpha 1 beta 1 can serve as a receptor for laminin-1 and collagen in certain cell types, but is a receptor for only collagen in others. To examine the molecular basis of this difference in specificity, three cell types were transfected with cDNA for the rat alpha 1 subunit. Following transfection with rat alpha 1, pluripotential hematopoietic human K562 cells exhibited alpha 1 beta 1-dependent attachment to collagen IV, but not laminin-1, unless activating antibody TS2/16 was added. The attachment to collagen IV stimulated the elaboration of a spread morphology resembling a differentiated megakarocyte with extensive processes which were absent in response to all other substrates. When MRC-5 cells, a human fibroblastic cell, or RD cells, a human rhabdomyosarcoma line, were transfected with the identical alpha 1-integrin construct, rat alpha 1 beta 1-dependent attachment to both collagen IV and laminin-1 was seen. Therefore differences in ligand specificity can be generated by translation of an identical integrin alpha 1 beta 1 mRNA in different cell types. Despite differences in ligand binding, alpha 1 cDNA-transfected K562 an...Continue Reading

References

May 1, 1992·The Journal of Cell Biology·A G ArroyoF Sánchez-Madrid
Jan 1, 1991·Annual Review of Neuroscience·L F Reichardt, K J Tomaselli
Oct 1, 1990·Experimental Cell Research·D GullbergK Rubin
Aug 1, 1990·The Journal of Cell Biology·M J IgnatiusL F Reichardt
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P GunningL Kedes
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M J Elices, M E Hemler
Apr 1, 1988·Immunology Today·M E Hemler
Aug 13, 1985·Biochemistry·A L TarentinoT H Plummer
Jan 1, 1982·Methods in Enzymology·R TimplG R Martin
May 4, 1995·International Journal of Cancer. Journal International Du Cancer·S S VeigaR R Brentani
Oct 1, 1994·Current Opinion in Cell Biology·T A Haas, E F Plow
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R ChammasR R Brentani
Oct 1, 1992·Trends in Cell Biology·M A Schwartz

❮ Previous
Next ❯

Citations

Dec 24, 2002·The Journal of Biological Chemistry·Wan-Ming ZhangDonald Gullberg
Feb 12, 1998·The Journal of Biological Chemistry·G Chi-RossoV E Koteliansky
Mar 6, 2002·Progress in Histochemistry and Cytochemistry·Donald E Gullberg, Evy Lundgren-Akerlund

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.