Heterologous expression of Deinococcus geothermalis amylosucrase in Corynebacterium glutamicum for luteolin glucoside production

Enzyme and Microbial Technology
Young-Wook ChinDong-Ho Seo

Abstract

Amylosucrase (ASase) has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization. In the present study, the properties of Deinococcus geothermalis ASase (DGAS) expressed in Corynebacterium glutamicum (cDGAS) and purified via Ni-NTA affinity chromatography were compared to those of DGAS expressed in Escherichia coli (eDGAS). The pH profile of cDGAS was similar to that of eDGAS, whereas the temperature profile of cDGAS was lower than that of eDGAS. The melting temperature of both enzymes did not differ significantly. Interestingly, polymerization activity was slightly lower in cDGAS than in eDGAS, whereas luteolin (an acceptor molecule) transglucosylation activity in cDGAS was 10 % higher than that in eDGAS. Analysis of protein secondary structure via circular dichroism spectroscopy revealed that cDGAS had a lower strand/helix ratio than eDGAS. The present results indicate that cDGAS is of greater industrial significance than eDGAS.

References

Oct 26, 1999·Current Biology : CB·N Pfanner
Apr 18, 2001·The Journal of Biological Chemistry·L K SkovM Gajhede
Oct 23, 2003·The Journal of Biological Chemistry·Cécile AlbenneMagali Remaud-Simeon
Mar 1, 2005·FEBS Letters·Sandra Pizzut-SerinMagali Remaud-Simeon
Apr 5, 2007·Nature Protocols·Norma J Greenfield
Oct 13, 2007·Proteins·Pascal BenkertDietmar Schomburg
Nov 25, 2011·Enzyme and Microbial Technology·Hyun-Kug ChoCheon-Seok Park
Feb 9, 2012·Applied Microbiology and Biotechnology·Dong-Ho SeoCheon-Seok Park
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Minghao GuoMartin Gruebele
Dec 3, 2014·European Journal of Pharmacology·Kyun Ha KimJaeho Cha
Feb 26, 2015·Critical Reviews in Biotechnology·Xiuxia LiuZhonghu Bai
Jul 23, 2015·Journal of Biotechnology·Heike OverwinBernd Hofer
Sep 13, 2015·Brain Research Bulletin·Seyed Fazel NabaviSeyed Mohammad Nabavi
Dec 9, 2015·Journal of Bacteriology·Melissa E Reardon-Robinson, Hung Ton-That
Mar 20, 2016·Enzyme and Microbial Technology·Dong-Ho SeoCheon-Seok Park
Nov 13, 2018·Frontiers in Microbiology·Min Ju Lee, Pil Kim
Mar 1, 2019·Food Science and Biotechnology·Eun-Ryoung KimCheon-Seok Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.