Heterologous expression of G-protein-coupled receptors

Trends in Biotechnology
C G Tate, Reinhard Grisshammer

Abstract

G-protein-coupled receptors (GPCRs) are integral membrane proteins of great pharmacological importance owing to their central role in the regulation of cellular responses to external stimuli. Heterologous expression systems have been used to explore ligand binding, G protein and effector coupling, and structural aspects of the receptors. GPCRs can be expressed in a functional form in all expression systems, but with varying degrees of success because of differences in receptor and host cell characteristics. This article will discuss aspects related to the choice and suitability of expression systems for the intended analysis of GPCR properties.

References

Apr 1, 1992·Naunyn-Schmiedeberg's Archives of Pharmacology·M J Lohse
Dec 22, 1989·Science·M ParmentierG Vassart
Aug 1, 1995·Quarterly Reviews of Biophysics·R Grisshammer, C G Tate
Mar 15, 1994·European Journal of Pharmacology·G Y NgB F O'Dowd
Aug 1, 1994·Current Opinion in Biotechnology·T W Schwartz
Jul 1, 1994·British Journal of Pharmacology·N BlinA D Strosberg
Jan 1, 1994·Annual Review of Biochemistry·C D StraderR A Dixon
Jan 1, 1994·Journal of Receptor Research·K E MazinaT M Fong
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S KaushalH G Khorana
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·T GudermannG Schultz
Aug 1, 1996·The Biochemical Journal·J Tucker, R Grisshammer

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Citations

May 24, 2005·Journal of Structural and Functional Genomics·Elena DobrovetskyChristopher M Koth
Oct 8, 2005·Journal of Structural and Functional Genomics·Xun ZuoTauseef R Butt
Dec 19, 2003·Protein Expression and Purification·Mark A MillerMichael A Whitt
Jun 14, 2005·Protein Expression and Purification·Malika AkermounKaty L Gearing
Jan 14, 1998·Trends in Pharmacological Sciences·J M StadelD J Bergsma
Apr 9, 1998·Baillière's Clinical Endocrinology and Metabolism·J SandersB R Smith
Nov 14, 1997·Current Opinion in Biotechnology·C K Jayawickreme, T A Kost
Nov 20, 1998·Current Opinion in Biotechnology·H Reiländer, H M Weiss
Feb 19, 2000·Current Opinion in Biotechnology·K G Fleming
Jan 11, 2002·European Journal of Biochemistry·H Markus Weiss, Reinhard Grisshammer
Jul 12, 2011·Molecular & Cellular Proteomics : MCP·Anton SteenDirk Jan Slotboom
Jun 23, 2004·European Journal of Biochemistry·Venkata R P RatnalaWillem J DeGrip
Aug 13, 2009·Molecular Cancer Therapeutics·Liping XuRobert J Gillies
Feb 7, 2016·Progress in Retinal and Eye Research·Nazhat Shirzad-Wasei, Willem J DeGrip
Jul 13, 2012·Trends in Biochemical Sciences·Christopher G Tate
Sep 29, 2009·Biochemical and Biophysical Research Communications·Rajesh PrasadAmitabha Chattopadhyay
Jan 6, 2009·Protein Expression and Purification·Alla KorepanovaMark L Chiu
Sep 25, 2007·Chemistry and Physics of Lipids·Md Jafurulla, Amitabha Chattopadhyay
Aug 25, 2015·Protein Expression and Purification·Narges Yousefi-RadMahdi Habibi-Anbouhi
May 30, 2006·Protein Science : a Publication of the Protein Society·Prashen ChelikaniH Gobind Khorana
Mar 26, 2011·British Journal of Pharmacology·Michael M Morgan, MacDonald J Christie
Nov 5, 1999·Protein Expression and Purification·G J TurnerM C Betlach
Apr 26, 2005·Biophysical Journal·Bryan W BergerClifford R Robinson
Aug 2, 2003·Biochemical and Biophysical Research Communications·Yasuomi UranoTakao Hamakubo
May 7, 2011·Biotechnology Progress·Wilber Romero-FernandezDasiel O Borroto-Escuela
Nov 28, 2001·Protein Expression and Purification·B E BusuttilA G Frauman

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