Heterologous Expression of Laccase From Lentinula edodes in Pichia pastoris and Its Application in Degrading Rape Straw

Frontiers in Microbiology
Chanjuan LiuXianghui Zhao

Abstract

Rape straw cannot be efficiently degraded and utilized by ruminants due to its severe lignification and complex cross-linked structure between fiber and lignin. The laccases can catalyze the inter-unit bond cleavage in lignin substrates. Therefore, this study investigated the recombinant laccase from Lentinula edodes (LeLac) and its application in degrading rape straw. The LeLac was expressed using Pichia pastoris. It had the maximum activity at 60°C and pH 3.0 using ABTS as substrate and at 50°C and pH 4.0 using o-tolidine as substrate. The LeLac exhibited preferential oxidation of ABTS and featured resistance to high temperature, but relatively poor thermal stability. The LeLac activity could be strengthened by Cu2+ in dose-dependent manners. The LeLac could tolerate 15% of ethanol and methanol. The optimal pH for the lignin degradation of rape straw acid detergent fiber (ADF) by LeLac was 4.0. The LeLac could improve the cellulose enzymolysis of rape straw ADF by degrading its lignin. Relatively fewer lignin but more soluble phenols from original rape straw were removed by LeLac. The enhancement of enzymatic hydrolysis in original rape straw should be a combined result of polyphenols removal and lignin degradation caused by ...Continue Reading

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Citations

Mar 14, 2021·Environmental Science and Pollution Research International·Manisha Malhotra, Sunil Kumar Suman

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Datasets Mentioned

BETA
AB035409.1

Methods Mentioned

BETA
Protein
electrophoresis

Software Mentioned

GraphPAD
GraphPad Prism
SPSS

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