Heterologous expression of metK1-sp and afsR-sp in Streptomyces venezuelae for the production of pikromycin

Biotechnology Letters
Sushila MaharjanJ K Sohng

Abstract

Two regulator genes, metK1-sp and afsR-sp, from Streptomyces peucetius ATCC 27952 were heterologously expressed in S. venezuelae ATCC 15439, to produce 14-membered pikromycin antibiotics. The production of pikromycin was increased by 1.6-fold and 2.6-fold by the expression of metK1-sp and afsR-sp, respectively. The overexpression of metK1-sp and afsR-sp in S. venezuelae stimulated the expression of the pathway-specific regulatory genes, pikD and ketosynthase, as demonstrated by RT-PCR. The elevated transcripts of the pikD and ketosynthase genes were consistent with the enhanced production of pikromycin.

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Citations

Nov 9, 2012·Applied Microbiology and Biotechnology·Ju ChuYourong Li
Nov 13, 2012·Applied Microbiology and Biotechnology·Kozo Ochi, Takeshi Hosaka
Apr 13, 2013·World Journal of Microbiology & Biotechnology·Xuejin ZhaoYuan Song
Dec 14, 2011·Applied Biochemistry and Biotechnology·Sushila MaharjanJae Kyung Sohng
Oct 1, 2013·BioMed Research International·Amit Kumar ChaudharyJae Kyung Sohng
Aug 12, 2014·Applied Microbiology and Biotechnology·Nguyen Huy ThuanJae Kyung Sohng
Aug 20, 2015·Applied Microbiology and Biotechnology·Anaya Raj PokhrelJae Kyung Sohng
May 17, 2011·Microbiological Research·Sarita PaudelJae Kyung Sohng
Jan 13, 2015·Applied Biochemistry and Biotechnology·Dipesh DhakalJae Kyung Sohng
Feb 1, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Valery M DembitskyVladimir V Poroikov
Mar 11, 2018·Journal of Industrial Microbiology & Biotechnology·Jeong Sang YiByung-Gee Kim
Nov 10, 2010·Natural Product Reports·Haoran ZhangBlaine A Pfeifer
Dec 20, 2019·Applied Microbiology and Biotechnology·Qin LiuJing He
Dec 11, 2019·Applied Microbiology and Biotechnology·Hue Thi NguyenJae Kyung Sohng
Aug 15, 2019·Microbial Cell Factories·Dipesh DhakalRamesh Prasad Pandey
May 1, 2021·Journal of Industrial Microbiology & Biotechnology·Hee-Ju NahEung-Soo Kim
Sep 14, 2021·Journal of Zhejiang University. Science. B·Yefeng HuXiaoping Yu
Oct 5, 2010·Microbiological Research·Bashistha Kumar KanthJae Kyung Sohng

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