Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Frontiers in Microbiology
Kiira S VuoristoRuud A Weusthuis

Abstract

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

References

May 29, 1999·Applied and Environmental Microbiology·S PankeM G Wubbolts
Aug 29, 2006·Journal of Molecular Biology·Bernhard LohkampGunter Schneider
Jun 28, 2008·Applied Microbiology and Biotechnology·Shin KanamasaEnoch Y Park
Dec 3, 2010·Methods in Molecular Biology·Evelina AngovRyan M Mease
Jan 10, 2013·Bioresource Technology·Tobias Klement, Jochen Büchs
Feb 20, 2013·Frontiers in Microbiology·Matthias G SteigerMichael Sauer
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alessandro MichelucciKarsten Hiller
Jan 21, 2014·Microbial Cell Factories·Laura van der StraatLeo H de Graaff
May 27, 2014·Frontiers in Microbiology·Germán L Rosano, Eduardo A Ceccarelli
Sep 13, 2014·Applied Microbiology and Biotechnology·Antje HevekerlKlaus-Dieter Vorlop
Oct 4, 2014·Applied Microbiology and Biotechnology·Kiira S VuoristoRuud A Weusthuis
Nov 26, 2014·The Journal of General and Applied Microbiology·Shusuke OkamotoHitomi Ohara

❮ Previous
Next ❯

Citations

Apr 4, 2017·Journal of Biotechnology·Peiching ChangClaire R Shen
Dec 10, 2016·Journal of Industrial Microbiology & Biotechnology·Badal C Saha
Oct 6, 2016·Biotechnology Letters·Irem Avcilar-Kucukgoze, Zoya Ignatova

❮ Previous
Next ❯

Datasets Mentioned

BETA
KT273317

Methods Mentioned

BETA
genetic modification

Software Mentioned

OptimumGene TM
GenScript

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved