Heterologous Expression of Pseudomonas putida Methyl-Accepting Chemotaxis Proteins Yields Escherichia coli Cells Chemotactic to Aromatic Compounds

Applied and Environmental Microbiology
Clémence RoggoJan Roelof van der Meer

Abstract

Escherichia coli, commonly used in chemotaxis studies, is attracted mostly by amino acids, sugars, and peptides. We envisioned modifying the chemotaxis specificity of E. coli by expressing heterologous chemoreceptors from Pseudomonas putida enabling attraction either to toluene or benzoate. The mcpT gene encoding the type 40-helical bundle (40H) methyl-accepting chemoreceptor for toluene from Pseudomonas putida MT53 and the pcaY gene for the type 40H receptor for benzoate and related molecules from P. putida F1 were expressed from the trg promoter on a plasmid in motile wild-type E. coli MG1655. E. coli cells expressing McpT accumulated in chemoattraction assays to sources with 60 to 200 μM toluene, although less strongly than the response to 100 μM serine, but statistically significantly stronger than that to sources without any added attractant. An McpT-mCherry fusion protein was detectably expressed in E. coli and yielded weak but distinguishable membranes and polar foci in 1% of cells. E. coli cells expressing PcaY showed weak attraction to 0.1 to 1 mM benzoate, but 50 to 70% of cells localized the PcaY-mCherry fusion to their membrane. We conclude that implementing heterologous receptors in the E. coli chemotaxis network i...Continue Reading

References

Jan 1, 1974·Antibiotics and Chemotherapy·H C Berg, D A Brown
Mar 1, 1981·Journal of Bacteriology·M Kihara, R M Macnab
Jun 12, 2002·Current Opinion in Microbiology·Rebecca E Parales, Caroline S Harwood
Nov 27, 2002·Applied and Environmental Microbiology·Gunjan Pandey, Rakesh K Jain
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Hendrik Szurmant, George W Ordal
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hal AlperGregory Stephanopoulos
Dec 20, 2005·Journal of Molecular Biology·Paige DerrMark Goulian
Feb 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Roger P Alexander, Igor B Zhulin
Jan 1, 2008·Trends in Biochemical Sciences·Gerald L HazelbauerJohn S Parkinson
Jun 27, 2009·Molecular Microbiology·Claudine BaraquetCécile Jourlin-Castelli
Aug 19, 2010·The EMBO Journal·Victor Sourjik, Judith P Armitage
Jan 25, 2011·Molecular Microbiology·Kylie J WattsMark S Johnson
May 25, 2011·Environmental Microbiology·Jesús LacalJuan L Ramos
Dec 16, 2011·Current Opinion in Cell Biology·Victor Sourjik, Ned S Wingreen
Jun 30, 2012·Microbiology·M Karina Herrera SeitzClaudia A Studdert
Sep 18, 2012·Current Opinion in Biotechnology·Tino KrellJose Julio Ortega-Calvo
Aug 8, 2014·FEMS Microbiology Reviews·Inmaculada SampedroJane E Hill
Apr 19, 2015·Current Opinion in Biotechnology·Rebecca E ParalesJayna L Ditty
Jun 11, 2016·ACS Synthetic Biology·Shuangyu BiVictor Sourjik
Aug 30, 2016·Molecular Microbiology·Stella StylianidouPaul A Wiggins
Jan 15, 2017·Current Opinion in Biotechnology·Clémence Roggo, Jan Roelof van der Meer
Jan 15, 2017·Current Opinion in Biotechnology·Miguel A Matilla, Tino Krell
Nov 11, 2017·Environmental Microbiology·Clémence RoggoJan Roelof van der Meer

❮ Previous
Next ❯

Citations

Feb 24, 2021·Analytical Biochemistry·Anne BoyeldieuCécile Jourlin-Castelli
Jan 22, 2022·Environmental Science and Pollution Research International·Muhammad IbrarXuewei Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.